牛病毒性腹泻病毒感染牛外周血单核细胞对CD80和CD86 mRNA转录的影响  

Effects of bovine viral diarrhea viruses in vitro on transcription of CD80 and CD86 mRNA in bovine peripheral blood mononuclear cells

作　　者：韩猛立[1] 黄新[1] 钟发刚[1] 

机构地区：[1]新疆生产建设兵团绵羊繁育生物技术重点实验室,新疆石河子832000

出　　处：《中国兽医学报》2012年第3期378-382,共5页Chinese Journal of Veterinary Science

基　　金：国家"973"计划前期研究专项资助项目(2008CB117017);国家自然科学基金资助项目(30960286)

摘　　要：用非致细胞病变(noncytopathic,NCP)和致细胞病变(cytopathic,CP)型牛病毒性腹泻病毒(BVDV)感染临床健康BVDV检测阴性的荷斯坦奶牛外周血单核细胞(PBMC),利用实时荧光定量PCR技术对感染后共刺激分子CD80和CD86mRNA转录水平的变化进行定量分析。结果表明,在NCP型BVDV感染牛PBMC后CD80在4h(P<0.05)和12,24h(P<0.01)出现2次转录高峰,CD86在6h(P<0.05)出现转录高峰;CP型BVDV感染后,CD80在24h(P<0.05)出现转录高峰,CD86在6h(P<0.05)出现转录高峰。尽管CD80在NCP型BVDV感染后呈现较复杂的动态变化,但结果提示NCP型和CP型BVDV感染均可导致牛PBMC的共刺激分子CD80和CD86基因转录在感染早期明显受到抑制,PBMC的抗原呈递能力受到影响。The clinically healthy Holstein bovine tested negative for bovine viral diarrhea virus（BVDV） in peripheral blood mononuclear cells（PBMC） were infected with noncytopathic（NCP） and cytopathic（CP） BVDV.The changes levels of mRNA of CD80 and CD86 genes were analyzed using a real-time fluorescent quantitative PCR（real-time FQ-PCR）.The results showed that the transcription of CD80 mRNA exhibited two transcription high at 4 h（P0.05） and 12,24 h（P0.01） post-inoculation（PI）,and CD86 mRNA reached the highest level at 6 h（P0.05）PI in NCP BVDV-infected PBMC;then the mRNAs transcriptions of CD80 peaked at 24 h（P0.05）and CD86 peaked at 6 h（P0.05） in CP BVDV-infected PBMC with signifficant differences compared to that of the other PI.While the transcription on CD80 mRNAs witnessed more kinetic changes,it indicats NCP and CP BVDV could significantly supress the transcription of CD80-CD86 genes early during the infection,and the situation might weaken the antigen presentation of PBMC in the inoculated bovine.

关 键 词：牛病毒性腹泻病毒 外周血单核细胞 CD80 CD86 实时荧光定量PCR 

分 类 号：S852.65[农业科学—基础兽医学]