饲料中鸡源性成分PCR-DHPLC检测方法的建立  

Establishment of PCR-DHPLC assay for detection of chicken tissue in feedstuff

作　　者：王金玲[1] 贾金生[1] 李俊环[1] 张莹[1] 王芳[1] 高铁军 

机构地区：[1]沈阳出入境检验检疫局,辽宁沈阳110016 [2]锦州市义县动物卫生监督所,辽宁锦州121100

出　　处：《中国兽医学报》2012年第3期493-496,共4页Chinese Journal of Veterinary Science

基　　金：沈阳市大型科学仪器设备共享专项资助项目(2007共享012)

摘　　要：以鸡组织为材料,提取鸡组织的线粒体DNA,以鸡12SrRNA基因为靶基因设计引物,PCR反应扩增片段230bp;用13种畜禽和水产动物线粒体DNA验证该PCR引物的特异性,结果只有鸡线粒体DNA为阳性;PCR-DHPLC检测灵敏度在DNA水平上达到了2.04mg/L;在随机采集的22份饲料样品中,检出8份鸡源性成分样品。结果表明,本研究建立的PCR-DHPLC方法可特异、灵敏地对饲料中的鸡源性成分进行快速准确检测。By using chicken tissue as test material,selecting the mitochondrial DNA（mtDNA） 12S rRNA gene as marker gene,we designed capture probes,amplified a fragment of 230 bp.No cross-reaction was detected with other 13 animal mitochondrial DNA.The PCR-DHPLC had a detection limit of 2.04 mg/L genomic DNA for chicken tissue.22 feedstuff samples detected 8 chicken tissue positive.These results indicated that the PCR-DHPLC assay could be used for specific rapid detection of chicken tissue in feedstuff.

关 键 词：饲料 鸡源性成分 PCR-DHPLC 

分 类 号：S851.34[农业科学—预防兽医学]