三种农田土壤中氨氧化细菌amoA基因多样性比较分析  

COMPARATIVE ANALYSIS OF AMMONIA MONOOXYGENASE(amoA) GENE DIVERSITY IN THREE CULTIVATED SOILS

作　　者：汪峰[1,2,3,4] 曲浩丽[1] 丁玉芳[1] 孙波[3] 崔中利[1] 曹慧[1] 

机构地区：[1]南京农业大学农业部农业环境微生物工程重点开放实验室,南京210095 [2]南京农业大学资源与环境科学学院,南京210095 [3]中国科学院南京土壤研究所,南京210008 [4]中国科学院研究生院,北京100049

出　　处：《土壤学报》2012年第2期347-353,共7页Acta Pedologica Sinica

基　　金：国家自然科学基金项目(40871125;40871123)资助

摘　　要：比较分析中国东部不同季风气候区中海伦黑土(HL)、封丘潮土(FQ)和鹰潭红壤(YT)3种土壤氨氧化细菌amoA基因多样性。采用非培养方法直接从土壤中提取微生物总DNA,用氨氧化细菌amoA基因特异引物扩增总DNA,构建了3种土壤amoA基因文库,并对文库进行限制性长度多态性(RFLP)分析。HL、FQ和YT的amoA基因文库克隆数量分别为49、50和48个,相应的RFLP类型数为10、10和14个OTUs,其中有4个OTUs为三种土壤共有;YT中氨氧化细菌amoA基因多样性指数最高,FQ最低;HL和FQ群落的相似为70%,HL与YT的相似度为50%,而FQ和YT之间仅为42%,说明氨氧化细菌具有地理分布的规律:17个amoA基因序列可以被聚成6个cluster,分属Nitrosospira和Nitrosomonas两个属。三种农田土壤中均存在丰富的氨氧化细菌,表明氨氧化细菌在农田土壤氮素循环中具有重要作用。To analyze by comparison AOB amoA gene diversity of three cultivated soils, i. e. black soil in Hailun （HL）, chao soil in Fengqiu （FQ） and red soil in Yingtan （YT） which are located in different climatic zones in East China. Total DNA of soil microbe was extracted by the direct lysis method, and amplified with specific primers of AOB amoA gene, amoA gene libraries of the three soils were thus constructed and analyzed with the RFLP method for diversity. The number of clones in amoA gene library was 49, 50 and 48 in HL, FQ and YT respectively, and their correspond- ing number of OTU （Operational Taxonomic Unit） was 10, 10 and 14, of while 4 were common in all the three samples. YT was the highest in index of amoA gene diversity while FQ the lowest ; The similarity index between HL and FQ in AOB community was 70% , between HL and YT was 50% , and between FQ and YT was 42% , which indicates that AOB distribution has geographic characteristics. Seventeen AOB amoA gene sequences could be sorted into six clusters belonging to two genera, Nitrosospira and Nitrosomonas. All the three soils have an abundance of AOB, thus implying autotrophic AOB play a crucial role in nitrogen cycling in cultivated soils.

关 键 词：氨氧化细菌 AMOA基因 RFLP分析 农田土壤 微生物多样性 

分 类 号：Q938[生物学—微生物学]