高脂诱导的胰岛素抵抗对apoE-／-小鼠甘油三酯代谢相关基因的影响  

The effects of high fat-induced insulin resistance on gene expression involving triglyceride metabolism in ApoE^-/^-mice

作　　者：王葱[1] 杨刚毅[1] 李伶[2] 孙滨[2] 李钶[1] 

机构地区：[1]重庆医科大学附属第二医院内分泌科,400010 [2]重庆医科大学检验系临床生化教研室和教育部实验诊断重点实验室

出　　处：《中国糖尿病杂志》2012年第3期219-222,共4页Chinese Journal of Diabetes

基　　金：国家自然科学基金项目(30771037,81070640,30871199和30971388)

摘　　要：目的探讨高脂诱导IR对apoE-/-小鼠TG代谢相关基因的影响。方法健康雄性apoE-/-小鼠,随机分为普食组(NF组,n=10)和高脂喂养组(HF组,n=10)共喂养16周。采用实时荧光定量PCR测定组织TG代谢相关基因:脂肪细胞甘油三酯酶(ATGL),激素敏感性脂肪酶(HSL),过氧化物酶体增殖物激活受体γ(PPARγ)mRNA表达,同时测定了脂肪组织ATGL蛋白水平。结果高脂喂养组小鼠空腹血糖、血浆胰岛素、胆固醇、游离脂肪酸(FFA)和TG明显升高(P均<0.01);肝组织和脂肪组织ATGL和PPARγmRNA的表达水平明显降低(P<0.01和P<0.05);而HSL mRNA表达没有明显变化。此外,高脂喂养组小鼠脂肪组织ATGL蛋白水平也明显低于普食组(P<0.01)。结论高脂喂养apoE-/-小鼠产生了明显的IR,可能与TG代谢相关基因及其蛋白水平的表达下调有关。Objective To investigate the effects of high fat-induced insulin resistance(IR) on gene expression involving triglyceride(TG) metabolism in apoE-/-mice.Method Healthy male apoE-/-mice were randomly divided into normal-chow group(NF,n=10) and high-fat fed group(HF,n=10) and fed for 16 weeks.The mRNA expression of adipose triglyceride lipase(ATGL),hormone sensitive lipase(HSL) and peroxisome proliferator-activated-receptor-γ(PPARγ) were measured by quantitative real-time PCR.ATGL protein level in adipose tissues was determined by Western blot.Results Fasting blood glucose(FBG),plasma insulin,free fatty acids(FFA),TG,total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) were significantly elevated in HF group compared with NF group(all P<0.01).The mRNA levels of ATGL and PPARγ in hepatic and adipose tissues were significantly lower in HF group than in NF group(P<0.01 and P<0.05),and the ATGL protein levels in adipose tissues were also lower in HF group than in NF group(P<0.01).Conclusion The high-fat-diet-fed mice with apoE-/-develop insulin resistance,which may induce the mRNA and protein down-regulate expression of genes involving TG metabolism.

关 键 词：代谢相关基因 高脂喂养 甘油三酯酶 胰岛素抵抗 小鼠 过氧化物酶体增殖物激活受体 荧光定量PCR测定 激素敏感性脂肪酶 

分 类 号：R714.244[医药卫生—妇产科学]