GC法同时测定降香中4个成分的含量  

GC simultaneous determination of four compounds in Dalbergia odorfera

作　　者：赵祥升[1,2] 魏建和[3] 甘炳春[1,2] 孟慧[1,2] 冯锦东[1,2] 

机构地区：[1]中国医学科学院﹠北京协和医学院药用植物研究所海南分所,万宁571533 [2]海南省南药资源保护与开发重点实验室,万宁571533 [3]中国医学科学院药用植物研究所,北京100193

出　　处：《药物分析杂志》2012年第3期392-395,399,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家"十一五"科技支撑计划(NO:2007BA127B01)

摘　　要：目的:建立降香挥发油中反式-β-金合欢烯、β-没药烯、反式-橙花叔醇、金合欢醇的气相色谱含量测定方法。方法:色谱条件为HP-5 MS(30 m×0.25 mm,0.25μm)色谱柱,程序升温;进样口温度250℃,流速1.0 mL.min-1;分流比20∶1;FID检测器,温度250℃;载气:氮气。结果:反式-β-金合欢烯、β-没药烯、反式-橙花叔醇、金合欢醇的线性范围分别为0.011～0.212 mg.mL-1(r=0.9998),0.011～0.659 mg.mL-1(r=0.9999),0.051～10.200 mg.mL-1(r=0.9996),0.011～0.222 mg.mL-1(r=0.9998);加样回收率(n=9)分别为99.5%,98.4%,99.7%,96.6%。结论:该方法简便、准确,重复性好,为降香的质量控制提供了依据。Objective：To develop a method for determination of trans-β-farnesene,β-bisabolene,trans-nerolidol and farnesylalcohol in volatile oil of Dalbergia odorfera by gas chromatography.Methods： The sample was determined with capillary column of HP-5MS（30 m×0.25 mm×0.25 μm）,and FID detector with the detector temperature at 250 ℃.The injector temperature was set at 250 ℃,the flow rate was 1.0 mL·min-1;split injection was conducted with split radio of 20∶ 1.Nitrogen was used as carrier gas.Results： Trans-β-farnesene,β-bisabolene,trans-nerolidol and farnesylalcohol had good linearity in the ranges of 0.011-0.212 mg·mL-1（r=0.9998）,0.011-0.659 mg·mL-1（r=0.9999）,0.051-10.200 mg·mL-1（r=0.9996）,0.011-0.222 mg·mL-1（r=0.9998）,respectively.The average recoveries（n=9）were 99.5%,98.4%,99.7% and 96.6%,respectively.Conclusion：A rapid,sensitive,accurate and high recovery rate method was developed.This study can provide a scientific basis for the quality control of Dalbergia odorfera.

关 键 词：降香(花梨木 降香檀) 挥发油 中药多组分分析 反式-β-金合欢烯 β-没药烯 反式-橙花叔醇 金合欢醇 气相色谱法 

分 类 号：R917[医药卫生—药物分析学]