毛细管区带电泳法分离盐酸美沙酮对映体  

Enantiomeric separation of methadone hydrochloride by capillary electrochromatography*

作　　者：胡敏 曹全胜 姜红 

机构地区：[1]湖北省食品药品监督检验研究院,武汉430064

出　　处：《药物分析杂志》2012年第3期410-413,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家十一五科技支撑计划项目(2008BAI55B05-002)

摘　　要：目的:采用毛细管区带电泳法对盐酸美沙酮对映体进行了拆分。方法:比较了3种衍生化β-环糊精为手性添加剂的分离效果,对缓冲液的pH及浓度、手性添加剂的浓度、柱温、分离电压等方面进行了考察及优化。结果:确定采用75μm×75 cm未凃渍石英玻璃管柱,运行缓冲液为含50 mmol.L-1的Tris和10 mmol.L-1的HP-β-环糊精的水溶液(以磷酸调节pH至2.0),分离电压为25 kV,柱温25℃,检测波长为205 nm,结论:该方法简便、快速,在15 min内分离度可达到1.9。Objective：To establish a CE method for the separation of the enantiomers of methadone hydrochloride.Methods：Using 3 β-cyclodextrin derivatives as chiral selector,the conditions for the enantiomeric separation were optimized,which including the concentration of the chiral selector,the pH of the buffer,the concentration of the buffer,column temperature,voltage.Results：The separation was performed on a fused-silica tubing 75 μm×75 cm at 25 ℃,with 50 mmol·L-1 Tris-10 mmol·L-1的HP-β-CD buffer（adjust pH to 2.0 with phosphoric acid）as the mobile phrase.Samples were separated at 25 kV.The detection wavelength was set at 205 nm.Conclusion：It is a simple,rapid method,which the resolution of the enantiomers can up to 1.9 within 15 min.

关 键 词：吗啡类物质 美沙酮 对映体 消旋体 环糊精 手性添加剂 手性拆分 毛细管区带电泳 

分 类 号：R917[医药卫生—药物分析学]