亲水作用色谱-蒸发光散射法测定益母草中盐酸水苏碱的含量  

HILIC - ELSD determination of Stachydrine hydrochloride in Herba Leonuri

作　　者：陈碧莲[1] 祝明[1] 黄卫国[2] 郭增喜[1] 

机构地区：[1]浙江省食品药品检验所,杭州310014 [2]浙江中医药大学,杭州310053

出　　处：《药物分析杂志》2012年第3期502-504,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家"十一五"重大新药创制专项(课题编号:2009ZX09308-001)

摘　　要：目的:建立亲水作用色谱-蒸发光散射检测器联用测定益母草中盐酸水苏碱含量的方法。方法:采用HILIC柱(250mm×4.6 mm,5μm);乙腈-0.2%冰醋酸溶液(80∶20)为流动相;Alltech2000蒸发光散射检测器:漂移管温度80℃,气体流速2.0 L.min-1;流速0.5 mL.min-1。结果:盐酸水苏碱进样量在0.536～10.713μg范围内呈良好的线性关系,平均回收率为101.9%,RSD为1.4%。结论:此方法简便、灵敏度高,重现性好,可用于益母草的含量测定。Objective： To establish a HILIC-ELSD method for determination of Stachydrine hydrochloride in Herba Leonuri.Method： The separation was performed on a HILIC column（250 mm ×4.6 mm,5 μm）with acetonitrile-0.2% glacial acetic acid solution（80∶ 20）as mobile phase at a flow rate of 0.5 mL·min-1.An Alltech 2000 evaporative light-scattering detector（ESLD）was used as detector with gas flow rate of 2.0 L·min-1 and drift tube temperature of 80℃.Results： There was a good linear relationship with the range of 0.536–10.713 μg.The average recovery was 101.9%（RSD = 1.4%）.Conclusion： The method is simple,sensitive and reliable,it can be used as quantitative analysis method for Herba Leonuri.

关 键 词：亲水作用色谱(HILIC) 益母草 盐酸水苏碱 强极性化合物分析 标准改进 

分 类 号：R917[医药卫生—药物分析学]