1型糖尿病酮症酸中毒患儿缺氧诱导因子-1α与血管内皮细胞生长因子mRNA表达水平的变化  

Changes of Hypoxia-Inducible Factor-1α and Vascular Endothelial Growth Factor mRNA Levels in Type 1 Diabetes Mellitus and Ketoacidosis

作　　者：商跃云[1] 黄乐[1] 孙桂香[1] 吕玲[1] 赵彦[1] 

机构地区：[1]天津市儿童医院内分泌科,天津300074

出　　处：《实用儿科临床杂志》2012年第6期406-408,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨1型糖尿病(T1DM)酮症酸中毒(DKA)患儿缺氧诱导因子-1α(HIF-1α)与血管内皮细胞生长因子(VEGF)mRNA水平的变化。方法天津市儿童医院住院T1DM并DKA患儿30例,于确诊24 h内(DKA 1组)及DKA纠正后10 d(DKA 2组)采血,另选取同期住院的不伴感染、缺氧、肿瘤或结缔组织病的同年龄同性别患儿30例为对照组。实时荧光定量PCR(Real-time PCR)法测定其外周血CD4+T淋巴细胞HIF-1α与VEGF mRNA的相对表达水平。PCR产物行琼脂糖凝胶电泳鉴定特异性。采用SPSS 13.0软件进行统计学分析。结果 3组HIF-1α及VEGF mRNA相对表达水平比较差异均有统计学意义(Pa<0.01)。DKA1组HIF-1α及VEGF水平明显高于对照组,差异有统计学意义(Pa<0.01);DKA纠正后HIF-1α及VEGF水平恢复,差异有统计学意义(P<0.05,0.01),但直至DKA纠正后10 d(DKA2组)仍未恢复至对照组水平,差异有统计学意义(P<0.01,0.05)。Real-timePCR产物行琼脂糖凝胶电泳,产物位于预期位置,确定产物特异性。结论 T1DM并DKA患儿CD4+T淋巴细胞HIF-1α与VEGFmRNA水平升高,且DKA纠正后HIF-1α与VEGF mRNA水平不能恢复至正常,这可能与T1DM并发症的发生发展有关。Objective To explore the changes of hypoxia-inducible factor-1α（HIF-1α） and vascular endothelial growth factor（VEGF） mRNA levels in type 1 diabetes mellitus（T1DM） and ketoacidosis（DKA）.Methods Blood samples of 30 cases with T1DM and DKA,who were diagnosed and treated in Tianjin Children＇s Hospital,were collected in 24 hours after diagnosed（DKA 1 group） and 10 days after DKA were corrected（DKA 2 group）,30 cases of same age and sex and without infection,anoxia,tumor or connective tissue disease were selected as control group.HIF-1α and VEGF mRNA levels in CD4＋T cell were measured by real-time polymerase chain reaction（Real-time PCR） method.Specificity of PCR products were validated through agarose gel electrophoresis.The data were statistically analyzed by SPSS 13.0 software.Results There were statistical differences of HIF-1α and VEGF mRNA levels between the 3 groups（Pa0.01）.HIF-1α and VEGF levels in DKA1 group were obviously higher than those in control group（Pa0.01）,the levels decreased（P0.05,0.01） but still above the control group（P0.01,0.05）,even 10 days after DKA were corrected.Specificity of products of Real-time PCR were determined by agarose gel electrophoresis for their characteristic location.Conclusions HIF-1α and VEGF mRNA levels in CD4＋T cell of T1DM and DKA are higher than normal,and after DKA corrected the levels are irreparable,which may be contributed to accelerate complications in T1DM.

关 键 词：1型糖尿病 糖尿病酮症酸中毒 缺氧诱导因子-1Α 血管内皮细胞生长因子 

分 类 号：R725.8[医药卫生—儿科]