高速逆流色谱法从白芍中分离纯化五没食子酰基葡萄糖  

Isolation and Purification of Pentagalloylglucose from Radix Paeoniae Alba by High-speed Counter-current Chromatography

作　　者：宋学英[1] 殷志爽[2] 赵逸松[1] 王桥[1] 

机构地区：[1]首都医科大学化学生物学与药学院,北京100069 [2]北京双鹤药业股份有限公司,北京100012

出　　处：《天然产物研究与开发》2012年第3期303-306,344,共5页Natural Product Research and Development

基　　金：北京市中医药科技项目(JJ2008-005)

摘　　要：本文建立高速逆流色谱(HSCCC)方法,从白芍粗提物中分离纯化五没食子酰基葡萄糖。分别采用正己烷-乙酸乙酯-甲醇-水体积比0.5∶5∶1∶5及0.5∶5∶0.5∶5混合溶剂作为两相溶剂体系,上相为固定相,下相为流动相,转速为800 rpm,流速为2.0 mL/min,用HPLC检测及ESI-MS进行验证。经过两次HSCCC分离纯化,得到五没食子酰基葡萄糖纯度为95.7%。High-speed count-current chromatography （HSCCC） was developed for separation and purification of penta- galloylglucose （PGG） from crude extract of Radix Paeoniae Alba by the twice-step separation. The two of biphasic sys- tems were composed of n-hexane-ethyl acetate-methanol-water at a volume ratio of 0. 5 ： 5 ： 1 ： 5 and 0. 5 ： 5 ： 0.5 ： 5 in HSCCC ,respectively. The upper organic phase was as the stationary phase and the lower aqueous phase as mobile phase. The flow rate was at 2.0 mL/min and revolution speed was at 800 rpm. The effluent was monitored with a UV detector at 280 nm. The HSCCC fractions collected were analyzed by HPLC and the target compound of PGG was separated with a purity of 95.7%. In this study,the HSCCC technique was shown to be a powerful method to purify PGG from crude ex- tract of Radix Paeoniae Alba and could represent a new technological tool to be applicable to the preparation of PGG from this source of products.

关 键 词：高速逆流色谱 高效液相色谱 白芍 五没食子酰基葡萄糖 

分 类 号：R284.2[医药卫生—中药学]