多药耐药鲍氏不动杆菌获得性耐药相关基因与可移动遗传元件检测的指标聚类分析  

Index cluster analysis of mobile genetic elements and acquired resistance genes in multidrug-resistant Acinetobacter baumannii

作　　者：姜如金[1] 朱健铭 吴康乐[1] 

机构地区：[1]杭州市余杭区中医院检验科,浙江杭州311106

出　　处：《中华医院感染学杂志》2012年第5期890-893,共4页Chinese Journal of Nosocomiology

基　　金：杭州市卫生科技计划项目(2009B053);杭州市余杭区重大科技项目(2009001)

摘　　要：目的了解多药耐药鲍氏不动杆菌获得性耐药相关基因和可移动遗传元件携带状况,并分析两者的相关性。方法收集2008年11月-2009年12月住院患者送检标本中分离的鲍氏不动杆菌共30株,采用聚合酶链反应(PCR)的方法,分析48种β-内酰胺类、氨基糖苷类、喹诺酮类获得性耐药相关基因和13种可移动遗传元件遗传标记,并对检测结果作指标聚类分析。结果 30株鲍氏不动杆菌共检出3种获得性β-内酰胺类耐药基因,5种获得性氨基糖苷类耐药基因,1种抗菌制剂外排泵基因,6种可移动遗传元件的遗传标记;其余46种基因未检出。结论多药耐药鲍氏不动杆菌指标聚类分析显示,ADC型β-内酰胺酶基因和抗菌制剂外排泵基因adeB与tnpU、tnp513、IS26、ISaba9、intⅠ1等可移动遗传元件遗传标记高度相关。OBJECTIVE To investigate the distribution of acquired resistance genes and mobile genetic elements in multidrug-resistant Acinetobacter baumannii,and the correlation between them.METHODS From Nov.2008 to Dec.2009,30 strains of A.baumannii isolated from inpatients were collected.Then 48 kinds of acquired resistance genes to β-lactams,aminoglycosides,quinolones,and 13 kinds of genetic markers of mobile genetic elements were analyzed by PCR.Furthermore,index cluster analysis was performed.RESULTS Among 30 strains of A.baumannii,3 kinds of acquired β-lactam-resistance genes,5 kinds of acquired aminoglycoside-resistance genes,one kind of drug efflux gene,6 kinds of genetic markers of mobile genetic elements were detected.However,the rest 46 kinds of genes were not detected.CONCLUSION In this group of A.baumannii,index cluster analysis showed that beta-lactamase blaADC,drug efflux gene adeB highly correlates with genetic markers of mobile genetic elements like tnpU,tnp513,IS26,ISaba9,intⅠ1.

关 键 词：鲍氏不动杆菌 抗菌药物 可移动遗传元件 指标聚类分析 多药耐药 

分 类 号：R378[医药卫生—病原生物学]