茅苍术提取物对胃癌BGC-823和SGC-7901细胞增殖抑制作用研究  

Proliferation Inhibition Effect of Atractylodes lancea(Thunb.) DC.Extracts in Human Gastric Carcinoma BGC-823 and SGC-7901 Cells

作　　者：王庆庆[1] 欧阳臻[1] 赵明[1] 韩邦兴[1] 王文兵[2] 吴燕[1] 杨兵[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]江苏大学生命科学研究院,江苏镇江212013

出　　处：《中药新药与临床药理》2012年第2期152-156,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省中医药局科技项目(LZ09086);江苏大学学生科研立项项目(09A050)

摘　　要：目的探讨茅苍术提取物对胃癌细胞株BGC-823和SGC-7901的增殖抑制效应。方法采用不同浓度(0.625,1.25,2.55,10 mg.mL-)1茅苍术水提物处理胃癌BGC-823和SGC-7901细胞,采用四甲基偶氮唑盐(MTT)法检测药物对细胞的抑制作用;倒置显微镜下观察细胞的形态学改变;Hoechst 33342染色,荧光显微镜观察细胞凋亡形态;应用流式细胞仪观察凋亡过程中的细胞周期变化。结果茅苍术提取物对胃癌BGC-823和SGC-7901细胞增殖均有明显的抑制作用,且呈浓度和时间依赖性;光镜下可见凋亡细胞的形态学特征性改变;流式细胞仪检测表明经茅苍术提取物处理,BGC-823细胞周期阻滞于S期,SGC-7901细胞周期阻滞于G0/G1期。结论体外实验显示茅苍术提取物能抑制胃癌BGC-823和SGC-7901细胞增殖,可以诱导胃癌细胞凋亡。Objective To observe the effect of extracts of Atractylodes lancea （Thunb.） DC. on the growth of gastric cancer cell lines of BGC823 and SGC7901. Methods Gastric cancer cell lines BGC823 and SGC7901 were treat ed with A tractylodes lancea（Thunb.）DC, extracts at the concentrations of 0.625, 1.25, 2.55, 10 mg·mL1. Cell via bility was measured by MTT assay. Morphological changes of the cells were observed under invert microscope after staining with Hoechst 33342. Cell cycle was examined by PI fluorescence flow cytometry（FCM）. Results The water extract of A tractylodes lancea（Thunb.）DC, could inhibit the growth of BGC-823 and SGC-7901 cell lines in dosage and time dependant manner. Cells exhibited apoptosis in morphology. The cell cycle of BGC823 was blocked in S phase, and that of SGC7901 was blocked in G0/G1 phase. Conclusion The extract of A tractylodes lance a（Thunb.） DC. could inhibit the proliferation and induce the apoptosis of gastric cancer cell lines of BGC823 and SGC7901.

关 键 词：茅苍术提取物 胃癌细胞BGC-823 SGC-7901 增殖 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]