藤稔葡萄主枝环剥对果实着色及相关基因表达的影响  

Influence of Main-branch-girdling on Berry Coloring and Expression of Some Related Genes in‘Fujiminori’Grapevine

作　　者：郭磊[1] 王涛[2] 岳林旭[3] 房经贵[1] 陈济林 宋长年[1] 冷翔鹏[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]温岭市农业林业局,浙江温岭317500 [3]山东省轻工农副原料研究所,山东高密261500 [4]浙江省温岭市滨海葡萄专业合作社,浙江温岭317500

出　　处：《园艺学报》2012年第3期409-416,共8页Acta Horticulturae Sinica

基　　金：教育部新世纪优秀人才支持计划项目(NCET-08-0796);江苏省现代园艺科学优势学科建设工程专项

摘　　要：对藤稔葡萄主枝环剥促进其果实着色的效果以及对花青苷合成相关重要基因的表达等进行了分析。研究结果表明,主枝环剥不仅可提高果肉中可溶性固形物的含量,并且明显改善果实着色,使外观颜色加深,果皮花青苷含量增加,果面光泽明亮度(L*值)和颜色组分b*值下降,颜色组分a*值以及红色葡萄果实颜色指数(CIRG值)增大。环剥处理还明显促进了UFGT、MYBA1和MYBA2的表达,MYBA1和MYBA2于环剥处理后的第2周(花后63d)表达水平明显上调,相对表达量达到最高值。UFGT的表达水平与花青苷的积累趋势具有很好的一致性,环剥处理使UFGT的相对表达高峰提前1周左右。Trunks of grapevine‘Fujiminori’were girdled for investigation of the influence of girdling on berry coloration and the expression of three important genes which is related to anthocyanin synthesis. The results showed that trunk-girdling could improve the mass fraction of soluble solids in flesh,obviously improve berry coloration,increase the contents of anthocyanin. Girdling treatment could decrease L＊ and b＊ values much,and increase the a＊ and CIRG value. In addition,trunk-girdling obviously regulated the expression of UFGT,MYBA1 and MYBA2. The results demonstrated that the expression of MYBA1 and MYBA2 was induced after the first 2 weeks（63 days after flowering）of girdling and the relative expression reached the highest value. Interestingly,the expression of UFGT and the anthocyanin accumulation of fruit peel in a highly similar pattern,and the girdling treatment causes the UFGT gene expression a week in advance.

关 键 词：葡萄 环剥 品质 着色 

分 类 号：S663.1[农业科学—果树学]