甜瓜抗蔓枯病基因Gsb-4的分子标记  

A SSR Marker Linked to Gsb-4 Loci Resistance to Gummy Stem Blight in Melon

作　　者：王红英[1] 钱春桃[1] 娄丽娜[1] 娄群峰[1] 张永兵[2] 伊鸿平[2] 吴明珠[2] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京210095 [2]新疆农业科学院,哈密瓜研究中心,乌鲁木齐830000

出　　处：《园艺学报》2012年第3期574-580,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金新疆联合基金项目(U1178307)

摘　　要：以甜瓜(Cucumis melo L.)抗蔓枯病自交系PI482398(含抗蔓枯病基因Gsb-4)和感病自交系‘白皮脆’以及它们的F1、BC1P1、BC1P2、F2群体为材料,苗期进行蔓枯病菌(Didymella bryoniae)接种鉴定,结果表明甜瓜抗蔓枯病基因Gsb-4为单显性遗传。利用集团分离分析法(bulked segregant analysis,BSA)对89对SSR引物进行筛选,引物CMTA170a在抗性材料中可扩增出约为120bp的条带,并与抗性基因Gsb-4表现出连锁关系。统计了CMTA170a在118个F2单株上的多态性,并利用MAPMAKER/Exp version3.0b软件进行了计算,其与Gsb-4的遗传连锁距离为5.14cM。The effects of 5-azacytidine（5-azaC）on DNA methylation levels and heat tolerance of two commercial cultivars of non-heading Chinese cabbage [Brassica campestris L. ssp. chinensis（L.） Makino],e.i‘.Xiadi’and‘Dongshangwei’,were studied. The results showed that DNA methylation levels of seedlings of the two cultivars were significantly decreased,no abnormal growth or development was observed. Pre-treatment of seeds of non-heading Chinese cabbage with 0.1–0.2 mol · L-1 of 5-azaC would slow down the decreasing of growth,POD activity,and soluble protein content of seedlings under heat stress,and decrease MDA content and cell membrane permeability as well.

关 键 词：甜瓜 蔓枯病 Gsb-4 SSR 

分 类 号：S634.1[农业科学—蔬菜学]