盐芥谷胱甘肽过氧化物酶基因(ThGPX6)的克隆及表达分析  

Cloning and Expression Analysis of Glutathione Peroxidase 6(ThGPX6)in Thellungiella halophile

作　　者：马亭亭[1] 周宜君[1] 高飞[1] 赵竹[1] 隋欣[1] 刘楠[1] 刘冉[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《植物遗传资源学报》2012年第2期252-258,270,共8页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(31070361);中央高校基本科研业务费专项基金(0910KYZY43;1112KYQN31);中央民族大学"985工程"项目(MUC98504-14;MUC98507-08);国家民委科研项目(10ZY01)

摘　　要：谷胱甘肽过氧化物酶在植物响应盐胁迫中具有重要作用。依据盐芥EST序列进行RACE实验,获得1个新的谷胱甘肽过氧化物酶基因,命名为ThGPX6(GenBank注册号为FJ357244)。该基因的cDNA全长892 bp,包含1个长为702bp的开放读码框,编码234个氨基酸。生物信息学分析表明,该蛋白具有植物GPX的典型结构,即GPX催化活性区(NVASKCGLT)和标志性基序(ILAFPCNQF),以及PHGPX特有序列(KWNF(S/T)KFL)。实时荧光定量PCR分析结果表明,ThGPX6在盐芥叶片和根中表达,其表达受NaCl诱导,显示ThGPX6在植物高盐响应中发挥作用。亚细胞定位结果表明,ThGPX6存在于线粒体和内体中的可能性最大,预示着ThGPX6在清除ROS过程中起着重要作用。Glutathione nalysis on EST sequence of oxidase was discovered and cleotides,including a single peroxidase（GPX） plays an important role in response to salt stress in plant. Through the a- Thellungiella halophila, a novel gene exhibiting high homology with plant glutathione per- named as ThGPX6（GenBank accession number：F J357244）. ThGPX6 consists of 892 nu- 702bp opening reading frame which encodes a 234-amino acid peptide. Bioinformatics a- nalysis showed that ThGPX6 had typical structures of GPX, such as catalysis region （NVASKCGLT）, characteristic mo- tif（ILAFPCNQF） and PHGPX special sequence （KWNF （S/T）KFL）. Quantitative real-time PCR analysis revealed that the gene expressed in shoot and root, and could be induced by NaC1 in T. halophila. These results showed that the ThGPX6 played a crucial role under salt stress in T. halophila. ThGPX6 is most likely localized in the mitochondria and endosome, suggesting importence of ThGPX6 in the process of cleaning ROS.

关 键 词：盐芥 ThGPX6 基因克隆 表达模式分析 亚细胞定位 

分 类 号：Q943[生物学—植物学]