平榛NAC转录因子的分离及表达特性分析  

Cloning and Expression Profiling Analysis of a Transcription Factor ChNAC1 in Hazelnut(Corylus heterophylla Fisch.)

作　　者：赵天田[1] 陈新[1,2] 刘庆忠[2] 王贵禧[1] 梁丽松[1] 马庆华[1] 

机构地区：[1]中国林业科学研究院林业研究所/林木遗传育种国家重点实验室,北京100091 [2]山东省果树研究所,泰安271000

出　　处：《植物遗传资源学报》2012年第2期265-270,共6页Journal of Plant Genetic Resources

基　　金：国家"十一五"科技支撑计划(2006BAD01A1701);国家林业局"948"项目(2008204208);中国林科院林业所重点项目(ZD200913)

摘　　要：NAC转录因子是近十年来新发现的具有多种生物功能的植物特异转录因子,在植物生长发育、激素调节和抵抗逆境等方面发挥着重要的作用。本研究基于Solexa技术对平榛花芽转录组文库进行分析,结合RACE-PCR扩增,从平榛中克隆了一个与NAC类基因同源的cDNA序列ChNAC1,该序列长度为1154bp,具有长度为876bp的完整开放阅读框架,推测编码蛋白含有291个氨基酸,具有N-末端同源性较高且十分保守的NAC结构域和一个位于C-末端的高度可变区域。qRT-PCR分析表明,ChNAC1可以在4℃低温胁迫条件下上调表达,在4h时出现表达峰值。组织表达分析结果表明,ChNAC1在雄花序中表达最高,其次是花芽、树皮和种子。推测ChNAC1可能参与植物响应低温反应过程。ChNAC1基因的克隆及表达分析为进一步阐明和探讨平榛NAC转录因子的功能奠定了基础。NAC transcription factors are a family of functionally diverse proteins. These plant-specific NAC do- main genes play an important role in response to various stresses. A cDNA encoding the NAC-like gene homologue was isolated from hazelnut（ Corylus heterophylla Fisch. ）by RACE-PCR and designated ChNAC1 （GenBank Acces- sion No. HQ639415）. Sequence analysis showed that cDNA of ChNAC1 was 1154 bp long and contained a single open reading frame. The predicted ChNAC1 protein has 291 amino acids with an estimated molecular mass of 33.31 kD and an isoelectric point of 6.66 kD,qRT-PCR analysis showed that the expression of ChNAC1 was induced by low temperature and peaked at 4 h after exposed to low temperatures of 4~C. The transcripts of ChNAC1 appeared in many hazelnut tissues including male inflorescence,bark, flower bud and seeds,but mostly accumulated in male inflorescence. These results suggest that ChNAC1 may function in cold stress signal transduction pathway.

关 键 词：平榛 NAC 低温 时空表达 

分 类 号：S664.4[农业科学—果树学]