基于SSR标记的贵州薏苡种质资源遗传多样性分析  

Genetic Diversity Analysis of Jobs Tears Germplasm Resources by SSR Markers in Guizhou Provience

作　　者：郭银萍[1] 彭忠华[1] 赵致[1] 陈志兴 高翔[3] 刘胜传 谢云[1] 

机构地区：[1]贵州大学农学院,贵阳550025 [2]黔西南州农业局,兴义570001 [3]贵州省农业科学院,贵阳550025 [4]贵州省兴义市兴仁县烟草公司,兴仁562300

出　　处：《植物遗传资源学报》2012年第2期317-320,共4页Journal of Plant Genetic Resources

基　　金：贵州省年度攻关项目(黔科合NY字[2008]3034号)

摘　　要：利用SSR标记研究了22份薏苡种质的遗传多样性,用11对扩增带型稳定的SSR引物从供试材料中检测出105个等位基因变异,每对引物检测等位基因4～20个,平均9.55个。SSR引物的PIC介于0.3048～0.9238,平均多态性信息量为0.8255。利用UPGMA聚类分系法将供试自交系划分为4类,该划分结果与根据地理来源、种质系谱的分类结果基本一致。SSR分子标记辅助的种质改良是薏苡品种改良的重要途径。The genetic diversity of 22 Jobs tears germplasm were studied by simple sequence repeats （SSR） markers. 11 SSR primers giving stable amplified band pattern detected 105 alleles among the lines tested. The av- erage number of alleles per SSR locus were 9.55 with a range from g to 20. The value of polymorphism information content （PIC） for each SSR locus varied from 0. 3048 to 0. 9238 with average of 0. 8255. The 22 germplasm were divided into 4 groups by UPGMA method based on SSR fingerprinting. The clustered results were similar to that based on geograph-ical resource and germplasmic genealogy. SSR marker could be an accurate and reliable method to study diversity of Jobs tears germplasm. SSR marker assisted genetic improvement of Jobs tears germplasm would be an important way to breed new Jobs tears cuhivars.

关 键 词：薏苡 种质 遗传多样性 SSR 

分 类 号：S519[农业科学—作物学]