汽油尾气致慢性肺损伤的形态学改变研究  

Study on the morphological changes of the rats’ lung caused by exhausting of gasoline engine

作　　者：王晔[1] 刘妍彤[1] 薛灵抒[1] 李美[1] 罗建[1] 程薇波[1] 

机构地区：[1]四川大学华西公共卫生学院,四川成都610041

出　　处：《现代预防医学》2012年第6期1349-1351,1354,共4页Modern Preventive Medicine

基　　金：四川省科技攻关计划资助(2009SZ0166)

摘　　要：目的探讨汽油机尾气致肺脏慢性损伤的形态学改变。方法大鼠40只,随即分为8组,即对照组、1d、3d、1周、2周、4周、8周、12周,每组5只,将汽油机尾气通入装有实验大鼠的自制染毒容器中染毒,每天染毒2h,于1d、3d、1周、2周、4周、8周、12周分别处死实验大鼠,对肺组织进行HE染色、masson染色,显微镜下对肺组织进行观察。结果前2周以炎症反应为主,4周后可见肺组织开始出现成纤维细胞增生,随时间延长,纤维组织增生也越来越明显,但仍有慢性炎症反应。结论汽油机尾气长期染毒可致大鼠肺脏损伤,以纤维化和慢性炎症为主。OBJECTIVE To find the morphological changes of the rats lung that inhaled exhaust of gasoline engine. METH- ODS Forty SD rats were randomly divided into eight groups, that is, normal control group, one day group, three days group, two weeks group, four weeks group, eight weeks group and twelve weeks group were included five rats respectively. Rats of each group were put into a container that can input and output air so that the concentration of carbon monoxide and HC can maintain a certain level. Then the gasoline engine were started up, and the exhaust were put into the container, and monitored the level of CO and HC, made their levels at 100ppm and 2.5%. Thus the rats inhaled the exhaust of gasoline engine every two hours. All groups lasted one day, three days, one week, two weeks, four weeks, eight weeks and twelve weeks, respectively. All rats of each group were sacrificed at given time. The lungs were taken out, and were sliced and dyed by Masson solution or hematoxylin and eosin, then were observed by light microscope. RESULTS No changes can be observed in the lung of normal control group, hyperemia can be observed in one day group and three days group, and some lymphocytes and monocytes can be observed in one week and two weeks groups; Some fibroblasts hyperplasia can be observed in four weeks group, also the in- flammation response can be observed. As time went on, the hyperplasia were more serious, till twelve weeks the fibrosis can be observed at the interstitial, in particularly, around vessels and bronchia. CONCLUSION The exhaust of gasoline engine can damn the lung of rats and can cause fibrosis.

关 键 词：汽油机尾气 慢性损伤炎症纤维化 动物模型 

分 类 号：R563[医药卫生—呼吸系统]