羟基自由基溶液喷淋灭菌影响因素分析  

Analysis on the effect Factors for Killing Bacterium by the Spraying ·OH Solution

作　　者：蔡丽娇[1] 高金莹[1] 武建芳[1] 杨宪立[1] 张芝涛[1] 

机构地区：[1]大连海事大学环境工程研究所,辽宁大连116026

出　　处：《现代预防医学》2012年第6期1461-1463,1466,共4页Modern Preventive Medicine

基　　金：国家自然基金(50877005)

摘　　要：目的研究并分析羟基自由基溶液以载体定量喷淋方式杀灭细菌效果的影响因素,为完成羟基自由基溶液消毒灭菌装备化提供技术参数。方法实验选取大肠杆菌(Escherichia coli)ATCC8032、枯草芽胞杆菌黑色变种(Bacillus subilis var.niger)ATCC9732、肠道球菌(Enterococcus)实验室保存菌种为研究对象,按照模拟消杀防疫技术流程,通过改变溶液喷淋后的雾滴粒径、喷淋空间高度、喷淋密度以及控制有无有机干扰物的手段进行研究。结果当控制压力为0.41MPa,雾滴粒径约为1080μm,·OH溶液喷淋密度约为40μl/cm2,高度为40cm,菌悬液无培养基的条件下,·OH溶液可完全杀灭初始对数值为6.61的大肠杆菌,对初始对数值为5.88的枯草芽胞杆黑色变种杀灭效果也可达到《消毒技术规范》的要求。结论·OH溶液喷淋雾滴粒径和有无有机干扰物是影响·OH溶液载体喷淋杀灭细菌的主要影响因素。OBJECTIVE To research and analyze on the factors for killing Bacterium by the spraying.OH to provide the teehni- eat parameters for realization of hydroxyl solution equipment. METHODS According to the disinfection and quarantine technolo- gy, Bacillus subtilis var.niger ATCC9732, E.coli （Escherichia coli） ATCC8099 and Enterococcus （laboratory strain kept） were collected for research subjects. According to the simulation shoppers epidemic prevention technology, we could perform study on different droplet size, different height, different spray quantity and with or without medium impurities. RESULTS Un- der the condition of 0.41MPa, ·OH solution droplet of 1080μm, the spray density was 40pd/cm^2, and the height was 40cm. The .OH can completely kill the initial Logarithm value 6.61 without bacteria suspension medium. At the same time, the killing effect of 5.88 initial logarithm value of Bacillus subilis var.niger can can meet the requirements of ＇ sterilization technical speci- fications＇. CONCLUSION Both ＂OH solution droplet size and the presence of medium impurities are the key points to effect killing bacterium.

关 键 词：·OH溶液 大肠杆菌 枯草芽胞杆菌黑色变种 肠道球菌 载体定量喷淋 

分 类 号：R187.2[医药卫生—流行病学]