甲型H1N1流感病毒感染患者血清细胞因子水平变化的意义  

Significant of cytokine level changes in serum of H1N1 Influenza A Virus infected patients

作　　者：陈海英[1] 刘义华[1] 李轲[1] 陈双峰[1] 

机构地区：[1]聊城市人民医院中心实验室,山东聊城252000

出　　处：《现代预防医学》2012年第6期1506-1507,共2页Modern Preventive Medicine

摘　　要：目的探讨甲型H1N1流感患者血清细胞因子水平变化对早期诊断急性肺损伤的临床意义。方法采用ELISA法检测H1N1病毒核酸阳性患者血清中Ⅱ型肺泡细胞表面抗原(KrebsVondenLungen-6,KL-6)、肺表面活性蛋白D(SP-D)、转化生长因子β1(TGFβ1)和白介素8(IL-8)的含量;X线检测患者肺部影像学的改变。结果病例组与对照组比较,血清KL-6,SP-D,TGFβ1和IL-8含量差异有统计学意义(F=390.040,P=0.000;F=352.102,P=0.000;F=369.974,P=0.000;F=333.707,P=0.014);与轻症者比较,重症患者KL-6的升高较为显著,差异有统计学意义(P﹤0.01);X线结果证实,KL-6升高明显的重症患者双肺呈典型的炎症性变化。结论 KL-6的检测有利于早期诊断甲型H1N1病毒感染患者肺损伤及疾病进展。OBJECTIVE To study the clinical significant of cytokine level changes in H1N1 patients serum for early to diagno- sis acute lung injury. METHODS The concentration of Krebs Von den Lungen-6 （KL-6）, pulmonary surfactant protein-D （SP-D）, transforming growth factor-β 1 （TGFβ 1 ） and interleukin-8 （IL-8） were investigated by ELISA method. The change of lung imaging was detected by X rays. RESULTS As contrast to control groups, the concentration of KL-6, SP-D, TGFβ1 and IL-8 in serum of case groups had significant difference （F = 390.040, P = 0.000; F = 352.102, P = 0.000; F = 369.974, P = 0.000 and F = 333.707, P = 0.014）. KL-6 increasing was significant when contrast to mild case groups （P 〈 0.01 ）. X rays results indicated that the double lungs of H1N1 patients had typical inflammation changes. CONCLUSION The detection of KL-6 makes is helpful for early diagnosis of lung injury and disease progression.

关 键 词：甲型H1N1流感病毒 细胞因子 Ⅱ型肺泡细胞表面抗原 肺表面活性蛋白D 

分 类 号：R373.13[医药卫生—病原生物学]