MiR-155在脂多糖诱导的RAW264.7巨噬细胞炎症反应中的表达研究  

Expression of miR-155 in the Lipopolysaccharide-induced Inflammation of RAW264.7 Macrophages

作　　者：谭玉军[1] 姚庆收[2] 张如意[3] 刘秀珍[2] 刘凤[4] 

机构地区：[1]滨州医学院病理生理学教研室,山东烟台264003 [2]滨州医学院药学院,山东烟台264003 [3]滨州医学院附属医院,山东滨州256603 [4]滨州医学院护理学院,山东烟台264003

出　　处：《安徽农业科学》2012年第8期4564-4565,4591,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省教育厅科技计划项目(J08LG02;J10LF91);山东省科技发展计划项目(2009GG20002055)

摘　　要：[目的]探讨miR-155在脂多糖(LPS)诱导的RAW264.7巨噬细胞炎症反应中的表达及糖皮质激素(GCs)的干预影响。[方法]体外培养RAW 264.7巨噬细胞,分别用浓度为10.0、1.0、0.1μg/ml LPS刺激RAW 264.7巨噬细胞,于2、6、12、24、36 h 5个时间点收集上清用ELISA检测白介素-6(IL-6)蛋白浓度;实时定量-PCR检测miR-155在2、6、12、36 h 4个时间点的表达变化。[结果]IL-6在各浓度LPS处理组、各时间点其含量均高于对照组(CK);miR-155的表达在各时间点LPS组均高于LPS+GCs和CK。[结论]LPS可以诱导RAW264.7巨噬细胞的炎症反应,炎症反应时miR-155高表达,糖皮质激素可抑制miR-155的表达。[ Objective ] To investigate the expression of miR-155 in lipopolysaccharide （LPS）-induced inflammation of RAW264.7 maerophages and the interventional impact of glucocorticoid （GCs）. [ Method] RAW264.7 macrophages were cultured in vitro ,and then simulated respectively with 10.0,1.0 and 0. 1 Vtg/ml l.PS,the supernatant was collected after 2,6,12,24 and 36h to detect the interleukin -6 （IL-6） protein concentra- tion by ELISA,the expression of miR-155 at 2,6,12 and 36 h was determined with real-time quantitative- PCR. [ Result ] IL-6 concentrations of LPS in each treatment group at each time point were higher than that in control group; miR-155 expression in the LPS group at each time point was higher than the LPS ＋ GCs and the control group. [ Conclusion ] LPS could induce the inflammatory response of RAW264.7 macrophage, high exnression of miR-155 aDoeared with the inflammatory resoonse .while alucocorticoids inhibited the expression of miR-155.

关 键 词：MIR-155 脂多糖 巨噬细胞 炎症 

分 类 号：S852[农业科学—基础兽医学] Q956[农业科学—兽医学]