基于电化学方法的抗癌中草药密蒙花中芹菜素的研究  

Study on the Apigenin of Antitumor Chinese Herbal Herb FLOS BUDDLEJAE

作　　者：赵国欣[1] 赵明[1] 刘艳丽[1] 李领川[1] 王立萍[2] 冯冲[1] 

机构地区：[1]中州大学实验管理中心,河南郑州450044 [2]河南省食品药品检验所,河南郑州450000

出　　处：《安徽农业科学》2012年第9期5138-5139,5601,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究抗肿瘤中草药芹菜素在玻碳电极上的电化学行为。[方法]运用循环伏安法(CV)、差示脉冲伏安法(DPV)进行研究。[结果]芹菜素运用循环伏安法(CV)在B-R(浓度50%乙醇,pH9.0)缓冲溶液中有一不可逆的吸收波,该氧化过程受扩散控制。利用差示脉冲伏安法(DPV)研究发现在芹菜素浓度为5.0×10-6～9.0×10-5mol/L范围内,吸收峰电流与峰电位有良好的线性关系,检测限为1.5×10-6mol/L。根据差示脉冲伏安法(DPV)中吸收峰的峰电流与芹菜素浓度的线性关系建立了该药物的电化学检测方法,该方法不用预分离其他黄酮类化合物就能达到检测样品中芹菜素含量的目的。[结论]这是一种新型的芹菜素的检测方法。[ Objective ] To study the electrochemical behavior of antitumor herbal drug apigenin at glassy carbon electrode, l Methods ] Cyclic voltammetry （CV）, differential pulse vohammetry （DPV） were used in experiments. [ Results ] In CV, only one irreversible anodic peak with Ep = 0.580 V was appeared in 0.1 M B-R buffer solution （50% ethanol, pH 9：0 ）. The peak currents were linearly related with apigenin concentrations in a range from 5.0× 10 -6 M to 9.0 × 10 -5 M. And the detection limit was 1.5×10 -6 mol/L. A new electroanalytical method for this herbal drug was established according to this anodic peak. Using the established method without pre-separation, apigenin in herbal drug was determined with satisfactory results. [ Conclusion] This method is a new one to detect apigenin.

关 键 词：密蒙花(FLOS BUDDLEJAE) 芹菜素 电分析方法 

分 类 号：S567[农业科学—中草药栽培] R284.1[农业科学—作物学]