RP-HPLC-ELSD法测定白花丹野生药材根·茎·叶中胡萝卜苷的含量  

Content Determination of Daucosterol in Roots,Stems and Leaves of Wild Plumbago zeylanica L.by RP-HPLC-ELSD

作　　者：左旭[1] 戴先芝[2] 张吉仲[1] 刘圆[1] 

机构地区：[1]西南民族大学民族医药研究院,四川成都610041 [2]阿坝师范高等专科学校,四川汶川623002

出　　处：《安徽农业科学》2012年第9期5150-5151,共2页Journal of Anhui Agricultural Sciences

基　　金：四川省杰出青年学术技术带头人后续计划(2011JQ0051);四川省科技支撑计划(2011SZ0233);中央高校基本科研业务费专项资金项目优秀科研团队及重大孵化项目(11NZYTH02);阿坝师专校级重点课题(ASA11-31)

摘　　要：[目的]建立测定白花丹药材中胡萝卜苷含量的方法,并研究不同采收期的野生白花丹药材根、茎、叶中胡萝卜苷的含量变化情况。[方法]采用RP-HPLC-ELSD检测法。迪马色谱柱C18(250 mm×4.6 mm,5μm);柱温25℃;流动相为无水甲醇;体积流量为1.0ml/min;载气为氮气,漂移管温度65℃。[结果]在0.129～2.58μg/ml浓度范围内,胡萝卜浓度对数值与峰面积对数值呈良好线性关系,R为0.9994(n=7);平均回收率99.93%,RSD为0.212%(n=9)。叶中含量最高,根中含量最低,2、7、8月份含量最高,5、6月份含量最低。[结论]该方法快速、简便、稳定,为建立白花丹野生变家种的栽培技术规范打下基础。[ Objective ] To establish a method for determining the content of daucosterol and compare its content from roots, stems and leaves of wild Plumbago zeylanica L.. [ Method ] High performance liquid chromatography - evaporative light scattering detection （ RP - HPLC - ELSD） was used to determine the content of daucosterol in wild P. zeylanica. Diamondsil Cls （250 mm × 4.6 mm, 5 μm） was used with pure methanol as the mobile phase and the flow rate was 1.0 ml/min. The temperature of column was 25℃. The flow rate of nitrogen at 2.5 L/min and the temperature of drift tube was 65℃. [ Result] The calibration curve of daucosterol was linear with concentration in the range of 0. 129 to 2.58 μg/ml with r = 0. 999 4 （ n = 7）. The average recovery was 99.98 % with RSD of 0. 212% （ n = 9）. The content of daucosterol was the highest in leaves and lowest in roots. For each part, the content of daucosterol in P. zeylanica was the highest in February, July, August, and the lowest in May and June. [ Conclusion ] The method is rapid, simple, stable. And the results provide data for establishing the cultivat- ed technique of Plumbaginis zeylanicae L..

关 键 词：白花丹 胡萝卜苷 含量测定 反相高效液相色谱法-蒸发光散射检测法 

分 类 号：S567[农业科学—中草药栽培] R284.1[农业科学—作物学]