附子总生物碱的提取工艺研究  

Extraction Technology of Total Alkaloids from RADIX ACONITI LATERALIS PRAEPARATA

作　　者：刘卓[1] 晏星[1] 徐春霞[1] 袁小红[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《安徽农业科学》2012年第9期5199-5201,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省科技支撑项目(2008SZ0082)

摘　　要：[目的]研究附子总生物碱的提取工艺,优选最佳提取条件。[方法]以提取率、总生物碱收率和总生物碱纯度为指标,采用单因素试验法,考察提取溶剂、提取方法、提取温度、粉碎度、料液比、提取时间和提取次数对附子中总生物碱提取率的影响,并优选出最佳提取工艺条件。[结果]最佳提取工艺条件为:在15℃条件下,将附子粉按1∶10(g/ml)加入无水乙醇,冷浸3次,每次1 d;在此条件下,浸膏得率、总生物碱收率和总生物碱纯度分别为3.15%、0.64%和20.32%。[结论]该方法高效、低毒、经济、操作方便、工艺可控,较适合附子总生物碱的提取和工业化生产。[ Objective ] To determine the optimal extraction technology of alkaloids from the roots of RADIX ACONITI LATERALIS PRA- EPARATA. [ Method] Taking extraction rate, and the purity and yield of total alkaloid as the indices, effects of extraction solvent, extraction method, extraction temperature, extraction time,comminution degree, solid-liquid ratio and extraction times on the extraction rate of total alkaloids were studied. And the optimal extraction technology was selected. [ Conclusion ] The optimal extraction technology was as follows ： RADIX ACONITI LATERALIS PRAEPARATA powder was added into anhydrous alcohol with the ratio of 1：10 （g/ml）under 15℃. After cold leaching for 3 times with each time for 1 d, the dry extract rate, extraction rate of total alkaloid, and purity of total alkaloid were 3.15% ,0.64% and 20. 32% .resDectively. [ Conclusion] This method was efficient.low toxic, economical.convenient and can be used for industrial production.

关 键 词：附子 总生物碱 冷浸 

分 类 号：S567[农业科学—中草药栽培] R284.2[农业科学—作物学]