NaCl胁迫对菜用大豆种子膨大初期蛋白质表达的影响  

Effect of NaCl stress on protein expression of vegetable soybean seed at early filling stage

作　　者：王聪[1] 朱月林[2] 杨立飞[2] 杨恒山[1] 

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028042 [2]国家大豆改良中心,南京农业大学园艺学院,南京210095

出　　处：《植物营养与肥料学报》2012年第2期417-425,共9页Journal of Plant Nutrition and Fertilizers

基　　金：“十一·五”国家高技术(863)研究计划重大项目(2006AA10A110);国家转基因生物新品种培育科技重大专项(2009ZX08004-011B);内蒙古民族大学科研创新团队计划项目(NMD1003);内蒙古民族大学博士启动基金项目(BS217)资助

摘　　要：采用蛭石栽培,对开花后10 d的菜用大豆[Glycine max(L.)Merr.]进行100 mmol/L NaCl处理,利用双向电泳(Two-dimensional gel electrophoresis,2-DE)技术对胁迫5 d时的种子蛋白质进行分离,并对处理与对照2-DE图谱中蛋白质表达进行比较分析。在对照和处理的2-DE图谱上均检测到327个蛋白点,有28个差异表达蛋白,其中16个较对照显著上调,另外12个显著下调。利用基质辅助激光解吸离子化飞行时间质谱(Matrix-assistedlaser desorption ionization time-of-flight mass spectrometry,MALDI-TOF-MS)对其中6个丰度差异较大(丰度变化在2.5倍以上)的蛋白进行分析,通过数据库检索,从中鉴定出5个蛋白质,分别是大豆球蛋白前体G2、肌动蛋白、大豆球蛋白前体G2相似蛋白、类α-微管蛋白和Kunitz型胰蛋白酶抑制剂,并对这些蛋白质在NaCl胁迫下可能的作用进行了讨论。结果表明,NaCl胁迫对菜用大豆种子膨大初期的蛋白质代谢产生了显著影响,肌动蛋白、类α-微管蛋白和Kunitz型胰蛋白酶抑制剂可能参与了对NaCl胁迫的应答反应。Under vermiculite culture conditions, vegetable soybean [ Glycine max （ L. ） Merr. ] was treated with 100 mmol/L NaC1 at the 10th day after flowering. After the separation of proteins from the seeds at the 5th day of the NaC1 stress by two-dimensional gel electrophoresis （2 -DE ）, the differences in protein expression were analyzed between the NaCI treatment and the control. About 327 proteins are detected at each 2 -DE map, of which 28 proteins are found to be differentially expressed. Among these NaCl-stress-changed proteins, 16 proteins are up-regulated, while other 12 proteins are down-regulated significantly. Out of the 28 proteins, the abundances { JP2 of 6 proteins are changed more than 2. 5 times. The 6 proteins are analyzed by using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry （ MALDI - TOF - MS） and searched in the database which allows the identification of 5 spots. These identified proteins are Glycinin G2 precursor, actin, similar to GLYCININ G2 PRECURSOR, alpha-tubulin-like protein and Kunitz trypsin inhibitor. The protein metabolisms at early seed-filling of vegetable soybean of the identified proteins are influenced significantly under the NaCl stress, and actin, alpha-tubulin-like protein and Kunitz trypsin inhibitor might be involved in NaCl stress responses.

关 键 词：菜用大豆种子 NACL胁迫 双向电泳 差异表达蛋白 

分 类 号：S643.7[农业科学—蔬菜学]