里岔黑猪GDF11基因cDNA的克隆及组织表达谱分析  

Molecular Cloning and Expression Patterns of GDF11 Gene in Licha Black Pig

作　　者：邢晋祎[1] 戈新[2] 贾坤航[1] 王建华[2] 刘忠琛 李培培[2] 张宝珣[2] 

机构地区：[1]临沂大学生命科学学院,山东临沂276005 [2]青岛市畜牧兽医研究所,山东青岛266100 [3]里岔黑猪原种场,山东胶州266300

出　　处：《华北农学报》2012年第1期24-29,共6页Acta Agriculturae Boreali-Sinica

基　　金：青岛市基础研究计划项目(09-1-3-64-jch);临沂大学博士启动基金项目(BS08019)

摘　　要：生长分化因子11(Growth and differentiation factor-11,简称GDF11)是BMP/TGF-β超家族一员,编码一种在早期胚胎发育过程中起重要作用的骨形态发生蛋白,在确立骨骼模式中起重要作用。本研究旨在克隆里岔黑猪GDF11基因部分cDNA,并检测其在不同组织中的表达差异。结果表明,所克隆的猪部分GDF11基因序列为1 161 bp(GenBank:HQ657211),编码332个氨基酸,与人、小鼠、大鼠、牛、马、兔、斑马鱼GDF11氨基酸序列同源性分别为99.10%,99.10%,99.10%,99.40%,99.10%,98.80%,78.82%。荧光定量PCR分析结果显示,GDF11基因mRNA在14种组织中均有表达,总体趋势为:脊椎>背膘>膀胱>肺>脾>胆囊>肾>大肠>心>骨骼肌>肝>小肠>眼肌>胃。这为进一步研究里岔黑猪GDF11基因的结构和功能及其脊椎数发生的机理奠定了基础。GDFI 1, also known as bone morphogenetic protein 11 （ BMP11 ） , is a member of the transforming growth factor-β superfamily and BMPs subfamily. It is a secreted protein which is considered to be a key regulator of axial skeleton patterning during early embryogenesis. In this study, the partial cDNA of porcine GDF11 gene was cloned by RT-PCR and the expression patterns of GDFll gene were detected. Sequence analysis indicates that the sequences of porcine GDFI1 gene were 1161 bp in length（GenBank：HQ657211.2）. The cloned cDNA encoded a protein of 332 amino acid residues,which shared 99.10% ,99. 10% ,99. 10% ,99.40% ,99. 10% ,98.80% and 78.82% identity with those of human, mouse, rat, cattle, horse, rabbit and zebra fish, respectively. Expression pat- terns of GDF11 were detected in 14 different tissues by real time quantitative RT-PCR, and patterns of GDFIi gene, as a whole, tended to be spinal cord 〉 backfat 〉 urinary bladder 〉 lung 〉 spleen 〉 gallbladder 〉 kidney 〉 large intestine 〉 heart 〉 skeletal muscle 〉 liver 〉 small intestine 〉 longissimus Dorsi muscle 〉 stomach. These results pro- vide important information for further elucidating the molecular biology functions and mechanism of GDF11 gene.

关 键 词：里岔黑猪 GDF11基因 基因克隆 荧光定量RT-PCR 组织表达谱 

分 类 号：S828[农业科学—畜牧学] Q789[农业科学—畜牧兽医]