玫瑰黄链霉菌抗药性标记及其定殖和促生作用研究  

Antibiotic-resistance tag of Stremptomyces roseoflavus Men-myco-93-63,its colonization and effects on plant growth-promoting

作　　者：杨淑[1] 张艳杰[1] 李爱霞[1] 孟庆芳[1] 李亚宁[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《河北农业大学学报》2012年第1期51-55,61,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31171894);国家高技术研究与发展计划(863)(2011AA10A205);河北省自然科学基金项目(C2011204114)

摘　　要：选用腐霉利、多菌灵、甲基托布津、利福平、青霉素、链霉素、泰乐菌素、重铬酸钾等8种常用抗生素或杀菌剂,通过抗药性标记试验,建立了玫瑰黄链霉菌(Stremptomyces roseoflavus)Men-myco-93-63的抗药性标记,确定了重铬酸钾(0.06g/L)/青霉素(0.2g/L)为适合检测该菌株的标记药剂及浓度。通过获得的抗药性标记,结合菌落形态观察和16SrDNA序列分析,研究了Men-myco-93-63在番茄根际土壤中的定殖动态和对植株生长的影响。结果表明,该生防菌在番茄根际土壤中能够定殖,接菌15d后定殖量达到最高,为5.6×105 cfu/g,之后下降,到30d后检测不到Men-myco-93-63菌株的存在。当使用Men-myco-93-63的固体发酵物与土壤以体积比1∶400混合时,对番茄苗期生长有促进作用。In order to develop the antibiotic-resistance tag of Stremptomyces roseoflavus Men-myco-93-63, eight kinds of antibiotics or mierobieides were tested, including proeymidone, car-bendazim, thiophanate-methyl, rifabutin, penicillin, streptomycin, tylosin, potessium,anddichromate potassium. It was determined that potessium dichromate potassium with the con-centration of 60 mg/L and penicillin with the concentration of 200 mg/L are the desirable anti-biotic-resistance tag for the detection of Men-myco-93-63. Using developed tag, with the ob-servation of colony morphology and the sequence analysis of 16S rDNA, colonizing dynamics ofthe strain around the tomato rhizosphere and their effects on the plants growth were evaluated.Results show that Men-myco-93-63 can colonize around the tomato rhizosphere. The amount ofcolonization reached the peak at the 15th day after inoculatiori, and then declined step by step.There was no Men-myeo-93-63 detected 30 days after inoculation. Moreover, it was found thatMen-myco-93-63 showed growth-promoting effect on tomato seedling, if maturing the mix- ture of Men-myco-93-63 solid fermentation with soil in proportion of 1：400.

关 键 词：玫瑰黄链霉菌 MEN-MYCO-93-63 抗药性标记 定殖 促生 

分 类 号：S432.1[农业科学—植物病理学]