鸡注射外源卵黄抗体后血清抗体衰减规律的研究  

The dynamic change of chicken serum antibody after inject IgY

作　　者：薛晓阳[1] 董世山[1] 陈立功[1] 王迎春[1] 褚军[1] 刘颖[1] 肖静静[1] 

机构地区：[1]河北农业大学动物医学院,河北保定071001

出　　处：《河北农业大学学报》2012年第1期71-74,共4页Journal of Hebei Agricultural University

基　　金：河北省科技支撑计划项目(07220401D-01)

摘　　要：通过研究鸡注射卵黄抗体后血清中抗体衰减的规律,为临床利用卵黄抗体治疗疾病提供理论指导。选取母源抗体衰减至近消失的60d龄海兰灰公鸡随机分成对照组和试验组。试验组每只肌注2mL含有新城疫、禽流感H5亚型和H9亚型3种抗体成分的卵黄抗体,对照组注射等量生理盐水。在注射后不同时间检测血清中3种抗体滴度的变化。结果显示试验组新城疫抗体在1～8d均极显著高于对照组(P<0.01);试验组禽流感H5亚型抗体滴度在1～11d极显著高于对照组(P<0.01),注射抗体组禽流感H9亚型抗体滴度在1～14d极显著高于对照组(P<0.01)。注射卵黄抗体后1d血清抗体即显著升高,5～6d内抗体效价维持较高水平,然后逐步衰减。试验表明肌注卵黄抗体能够极显著提高鸡血清抗体滴度,注射后5d内抗体维持较高水平,半衰期大约为5d,之后随时间推移抗体滴度逐渐降低。证明肌注卵黄抗体治疗疾病具有起效快作用可靠的优点,值得临床推广应用。This study a at guidance tenuated for clinica to almost on the dynamic change of chicken antibody after inject IgY aims to provide 1 use of yolk antibody. 50 highland gray cocks whose maternal antibody disappear randomly assigned into 2 groups. The experiment group wereinjected with 2 mL IgY including ND-Ab, AVI-Ab H5 and AVI-Ab H9 while the control group with 2 mL Sodium Chloride. The chicken serum antibody titer was measured by hemagglutina- tion inhibition test at different time. Results. the ND-Ab titer was significantly higher thanthat of control group in 1-8 day（P〈0.01） ,AVI-Ab H5 titer was significantly higher than the control group in 1-11 day（P〈0. 01） ,and AVI-Ab H9 titer was significantly higher than the control group in 1-14 day（P〈0.01）. The serum antibody titers increased significantly just ldpost injection while the high antibody titers continued 5-6 days and then reduced gradually. The half life period of IgY in the serum was about 5days. It can be concluded that injecting the egg yolk antibody can significantly improve chicken serum antibody titer.

关 键 词：鸡 卵黄抗体 血清抗体 衰减 

分 类 号：S852.3[农业科学—基础兽医学]