亚硫酸钠对HepG2细胞毒性的实验研究  

Toxicity of Sodium Sulfite to HepG2 Hepatocytes

作　　者：赵春燕[1] 原福胜[1] 白剑英[1] 雷佩玉[1] 任玉龙[1] 

机构地区：[1]山西医科大学公共卫生学院环境卫生学教研室,山西太原030001

出　　处：《环境与健康杂志》2012年第3期203-206,F0003,共5页Journal of Environment and Health

基　　金：国家自然科学基金(30972445);山西省自然科学基金(2007011109)

摘　　要：目的观察食品添加剂亚硫酸钠对人肝肿瘤细胞(HepG2)的细胞毒性作用和脂肪变作用。方法向HepG2细胞悬液中分别加入终浓度为0(阴性对照)～10 mmol/L亚硫酸钠溶液染毒24、48和72 h,并设空白对照(无细胞)组和阳性对照组(含1%Tritonx-100的DMEM),每组6个复孔。采用乳酸脱氢酶(LDH)活力测定法检测肝细胞膜的通透性变化,采用CCK-8法检测肝细胞的活性改变,采用油红O染色法观察肝细胞内脂肪沉积情况。结果与阴性对照组相比,仅10 mmol/L亚硫酸钠染毒24 h后HepG2细胞培养上清中LDH活力的释放率增加(P<0.05);而各剂量亚硫酸钠染毒48和72 h后HepG2细胞培养上清中LDH活力的释放率无显著变化。与阴性对照组相比,各剂量亚硫酸钠染毒24、48、72 h后(除0.039 mmol/L染毒72 h外)HepG2细胞的存活率均显著下降(P<0.05),且细胞存活率随着亚硫酸钠染毒剂量的升高而降低。染毒24、48 h后,部分亚硫酸钠染毒组HepG2细胞内出现了极少量的脂滴,而阴性对照组却未发现;染毒72 h后,10 mmol/L亚硫酸钠染毒组出现明显脂滴。结论一定剂量的亚硫酸钠染毒可增加HepG2细胞膜的通透性,对肝细胞活性有明显的抑制作用,并且可引起肝细胞内甘油三酯的蓄积。Objective To observe the toxicity and fatty degeneration in human hepatoma cells （HepG2 cells） induced by food additive sodium sulfite. Methods The HepG2 cells were treated with different concentration of sodium sulfite （0-10 retool/L） and 1% Triton X-100 DMEM （positive control group） for 24,48 and 72 h. The activity of the actate dehydrogenase （LDH） was used to detect liver cell membrane permeability changes. CCK-8 method was used to detect cell viability and oil red 0 staining for fatty deposition detection. Results Compared with negative control group, 10 mmol/L sodium sulfite exposure for 24 h increased significantly the LDH activity of HepG2 cells cultural supematant （P〈0.05）, and no other dosages of sodium sulfite exposure for 24 h and all dosages exposure for 48 and 72 h changed significantly the LDH activity of cultural supernatant. Compared with the negative control group, HepG2 cell viability of all sodium sulfite treated groups,except for 0.039 mmol/L exposure for 72 h,were significantly decreased （P〈0.05）, and the survival rate of cells decreased with the increase of sodium sulfite exposure dose. When treated for 24 and 48 h, few amount of lipid droplets in some sodium sulfite exposure groups but no droplets could be found in thd negative control group; exposed for 72 h, 10 mmol/L sodium sulfite exposure group showed obvious lipid droplets. Conclusion Certain dose of sodium snlfite exposure can increase cell membrane permeability and inhibit cell viability and cause intracellular triglyceride accumulation in HepG2 cells.

关 键 词：亚硫酸钠 人肝肿瘤细胞 乳酸脱氢酶 甘油三酯 

分 类 号：R994.6[医药卫生—毒理学]