大鼠背根神经元中Ca2＋的激光共聚焦显微成像与定量分析  

Imaging and Quantitative Analysis of Ca^(2+) in Dorsal Root Ganglion Neurons by Confocal Laser Scanning Microscopy

作　　者：沈秀球[1] 黄义梅[1] 杨洪钦[1] 郑莉琴[1] 王瑜华[1] 谢树森[1] 

机构地区：[1]医学光电科学与技术教育部重点实验室,福建师范大学激光与光电子技术研究所,福建福州350007

出　　处：《福建师范大学学报（自然科学版）》2012年第2期66-70,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(60978071);福建省自然科学基金资助项目(2010J01322)

摘　　要：成功实现体外培养背根神经元(Dorsal Root Ganglion,DRG),利用差速贴壁法进行提纯,通过细胞免疫荧光技术鉴定所培养的细胞纯度,发现其纯度高达90%,完全适合运用于细胞分子机理研究.在此基础上,采用激光共聚焦显微成像实验观察DRG内的Ca2+荧光信号,并对Ca2+浓度进行了定量分析.研究结果有助于深入探究和定量分析神经元中Ca2+参与特定的细胞生理功能.Dorsal root ganglion （DRG） neurons were successfully cultured and purified by differential adhesion method. The purity of the DRG neurons were identified with a pu- rification rate up to 90~ via the cellular immunofluorescence technique, which is suitable for studying the related cell molecular mechanism. The calcium fluorescence signals from cul- tured DRG neurons were investigated by the confocal laser scanning microscopy. And then the concentration of calcium in DRG neurons was quantitatively analyzed. The results are useful for further exploration and quantitatively analyzing the calcium involving the specific cell physiological functions of neurons.

关 键 词：背根神经节(DRG) 神经元 疼痛 CA2+ fluo-3/AM 

分 类 号：R318.51[医药卫生—生物医学工程]