奶牛乳腺蛋白组分析中蛋白质分步提取方法的建立  

Establishment of Sequential Extraction Method in the Proteome Analysis of Mammary Gland from Dairy Cows

作　　者：秦芝蕾[1,2] 左谦益[2] 伏小平[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]兰州生物制品研究所有限责任公司实验动物室,甘肃兰州730046

出　　处：《动物医学进展》2012年第3期66-68,共3页Progress In Veterinary Medicine

摘　　要：全息蛋白质样品提取是蛋白组研究的先决条件,采用3种溶解性质不同的裂解液分步提取奶牛乳腺蛋白组,分别进行双向聚丙烯酰胺凝胶电泳分离,PDQUest 7.4分析凝胶图谱,发现其与常规方法相比,具有蛋白质提取率高、双向电泳(2-DE)分辨率高等优点。The total sample preparation is a prerequisite for proteomic analysis.A three-step sequential protein extraction method was applied in proteomics analysis：using three kinds of solutions with differential solubility to replace one kind of solution.Proteins were separated by IEF and SDS-PAGE.And the patterns were analyzed with PDQUest 7.4 software.The method improved the efficiency of extraction and separation of the proteins compared with method of one solution.

关 键 词：分步提取 双向电泳 蛋白组 

分 类 号：S854.43[农业科学—临床兽医学]