Calpain活化在H_2O_2诱导的成年小鼠心肌细胞损伤中的作用  

The Role of calpain activation in cardiomyocyte injury induced by H_2O_2 in mice

作　　者：岳荣川[1] 胡厚祥[1] 罗涛[1] 李科[1] 张双[1] 徐磊[1] 刘利[1] 雷蕾[1] 

机构地区：[1]川北医学院附属医院心血管内科,四川南充637000

出　　处：《西部医学》2012年第3期426-429,共4页Medical Journal of West China

基　　金：国家自然科学基金(No:81070101);四川省科技厅课题(No:2009JY0079)

摘　　要：目的探讨Calpain(钙蛋白酶)在ROS介导的心肌细胞损伤中的作用。方法原代培养的成年小鼠心肌细胞分为3组:对照组;H2O2组(用25μM H2O2处理原代培养的成年小鼠心肌细胞3h来模拟ROS介导的心肌细胞损伤);PD+H2O2组,H2O2处理前用20μM PD150606(Calpain抑制剂)预处理心肌细胞30min。检测各组细胞的存活率、Calpain活性、caspase-3活性、细胞色素C的释放以及Ca2+浓度的变化情况。结果与对照组相比较,H2O2组的细胞存活率下降;Calpain及caspase-3活性增高,细胞色素C的释放以及细胞内Ca2+均明显增加(P<0.05)。然而,用PD150606预处理细胞30min能抑制H2O2引起的上述变化。结论 Calpain活化在ROS介导的心肌细胞损伤中可能具有重要作用。Objective To investigate the role of Calpain in reactive oxygen species（ROS）-mediated cardiomyocyte injury.Methods Primary cultured adult mouse cardiomyocytes were randomly divided into the three different groups： a control group;the H2O2-treated group in which cardiomyocytes were treated with 25μM H2O2 for 3 hours;and the PD＋H2O2 group in which cardiomyocytes were pretreated with 20μM PD150606,a Calpain inhibitor,for 30min prior to H2O2 treatment.Cardiomyocyte survival,Calpain activity,caspase-3 activity,cytochrome c release,and intracellular Ca2＋ concentrations were determined in these groups.Results In the H2O2-treated group,the survival of cardiomyocytes significantly decreased,and the activity of Calpain and caspase-3,as well as cytochrome c and Ca2＋ concentrations were significantly increased compared to the control group（p0.05）.However,these effects of H2O2 on cardiomyocytes were alleviated by pretreatment with PD150606.Conclusion Calpain activation may play an important role in ROS-mediated cardiomyocyte injury.

关 键 词：钙蛋白酶 活性氧化产物 心肌细胞 

分 类 号：Q331[生物学—遗传学]