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作 者:刘远[1] 李文杨[1] 林仕欣[1] 张晓佩[1] 高承芳[1] 林碧芬[1] 董晓宁[1]
机构地区:[1]福建省农业科学院畜牧兽医研究所,福建福州350013
出 处:《家畜生态学报》2012年第1期19-22,42,共5页Journal of Domestic Animal Ecology
基 金:福建省公益类科研院所基本科研专项(2011R1025-9);福建省农业科学院青年人才创新基金项目(2010QC-14)
摘 要:以控制部分绵、山羊品种高繁殖力的BMPR-IB、BMP15和GDF9基因为候选基因,采用PCR-RFLP方法分析闽东山羊BMPR-IB,BMP15和GDF9基因多态性与繁殖性状的关系。研究发现:多胎品种闽东山羊及南江黄羊在BMPR-IB基因的相应位置上并未发生与Booroola Merino羊相同的突变,同时也未检测到BMP15的FecXI,FecXH,FecXB基因及GDF9的FecGH基因,因此排除了这5个突变位点影响闽东山羊高繁殖力性状的可能性。然而,由于闽东山羊的BMPR-IB、BMP15、GDF9的全基因序列信息的缺乏,尚不能完全断定3个基因对闽东山羊高繁殖力性状没有影响。BMPR-IB, BMP15 and GDF9 were studied as candidate genes,which control the fecundity of sheep and goat PCR-RFLP was applied to analyze the relationship between the gene and genotype polymor-phisms of BMPR-IB, BMP15 and GDF9 in Mindong Goat and their prolific trait. The results showed that there was no mutation in BMPR-IB gene of Mindong Goat as that of Booroola Merino. The FecXI, FecXH , FecXB of BMP15 and FecGH of GDF9 were not found. Then it was ruled out the possibility that the re- production fecundity of Mindong Goat were affected by the mutations of BMPR-IB, BMP15 and GDF9 gene. As the whole gene sequence of the three genes has not been revealed in Mindong Goat, it's hard to determine whether these genes affect the fecundity of Mindong Goat.
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