葡萄组织培养中愈伤组织的诱导  

Callus Induction in Tissue Culture of Grape

作　　者：崔鹏[1,2] 高秀宇[1] 李波[1,2] 吴月燕[1] 

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100 [2]浙江大学农业与生物技术学院,杭州310029

出　　处：《湖北农业科学》2012年第4期827-830,共4页Hubei Agricultural Sciences

基　　金：宁波市重大科技计划项目(2009C10003);宁波市科技攻关项目(2008C1002);浙江万里学院重中之重学科开放基金项目(KF2010003)

摘　　要：采用三因素(萘乙酸NAA,吲哚丁酸IBA,6-苄氨基嘌呤6-BA)四水平的正交试验设计,对"鄞红"葡萄近梢处的嫩茎段和幼果进行愈伤组织诱导试验。结果表明,6-BA对愈伤组织诱导影响最大。以嫩茎段为外植体的最佳培养基配方是MS+1.00 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L IBA,其诱导率为70.00%。以幼果为外植体的最佳培养基配方是MS+0.80 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L IBA,诱导率为61.85%。嫩茎段是"鄞红"葡萄愈伤组织诱导最理想的外植体。L16（43） orthogonal design was used to study the effects of 3 factors（NAA,IBA,6-BA） on callus induction from tender stem segments and young fruit of ＂Yinhong＂ grape.The results showed that the main factor influncing callus induction was 6-BA.The optimum callus induction medium for tender stem segments was MS＋1.00 mg/L 6-BA＋0.10 mg/L NAA＋0.20 mg/L IBA,in which the induction rate could reach to 70.00%.The optimum callus induction medium for young fruit was MS＋0.8 mg/L 6-BA＋0.20 mg/L IBA ＋0.10 mg/L NAA,in which the induction rate was 61.85%.Tender stem was better explant than young fruit.

关 键 词：葡萄 嫩茎 幼果 愈伤组织诱导 正交设计 

分 类 号：S663.1[农业科学—果树学] Q813.12[农业科学—园艺学]