单核苷酸多态性电化学生物传感检测研究进展  

Research Progress of Electrochemical Biosensor for Single Nucleotide Polymorphisms Detection

作　　者：封科军[1] 李继山[2] 蒋健晖[2] 沈国励[2] 俞汝勤[2] 

机构地区：[1]惠州学院化学工程系,广东惠州516007 [2]湖南大学化学化工学院化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《分析测试学报》2012年第3期359-364,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21035001;20775023);广东省自然科学基金项目(10451601501006188);惠州学院博士启动基金项目(C509.0202)

摘　　要：作为第3代遗传标记物,单核苷酸多态性分析在实现遗传疾病的早期医学诊断以及发病机理研究等方面具有非常重要的意义,已成为当今分析化学和生命科学研究的热点领域。电化学生物传感器因具有灵敏、检测装置轻便价廉且易于微型化、自动化等优点,近年在单核苷酸多态性检测分析中得到了迅速的发展。该文简述了单核苷酸多态性生物传感检测的识别原理、电化学生物传感检测方法及信号放大策略,综述了近年来的研究进展,并对未来的发展方向作了展望。As the third generation of genetic marker, the analysis of single nucleotide polymorphisms （SNPs） has a great significance for the pathogeny and early medical diagnosis of gene diseases, and has become a hotspot of analytical chemistry and life science research. In recent years, the electro- chemical biosensors have been widely used in SNPs assay due to its advantages of high-sensitivity, simple-design, low-cost, easy miniaturization and automation. In this paper, the principle of SNPs typing, electrochemical biosensor technology and signal amplification methods for SNPs are intro- duced. Moreover, the current research progress on electrochemical detection for SNPs is summa- rized, and the future direction of SNPs electrochemical detection is discussed.

关 键 词：单核苷酸多态性 生物传感器 电化学检测 信号放大 研究进展 

分 类 号：O657.1[理学—分析化学] Q524[理学—化学]