茶多酚主要成分EGCG体外抗流感病毒FM1株作用  

Antiviral Effect of Tea Catechin EGCG on Influenza Virus FM1 Strain in Vitro

作　　者：谢慧珺[1] 刘妮[1] 丁伟[1] 赵昉[1] 史晨希[1] 徐培平[1] 赖小平[2] 张奉学[1] 

机构地区：[1]广州中医药大学热带医学研究所,广东广州510405 [2]广州中医药大学中药学院,广东广州510405

出　　处：《广州中医药大学学报》2012年第2期172-175,224,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金-广东省政府联合项目(编号:u0732004);国家自然科学基金项目(编号:81072948)

摘　　要：【目的】研究茶多酚主要成分EGCG体外抗甲型流感病毒FM1株感染及抑制病毒复制的作用。【方法】细胞培养测定抗病毒效价,采用细胞病变抑制(CPE)法和空斑减少试验法,在不同试验策略(治疗、保护)下,评价其体外抗流感病毒作用。【结果】CPE法观察及四甲基偶氮唑盐(MTT)法检测结果显示:在治疗及保护模式下,4～32 mg/mL EGCG均有显著抑制病毒、提高细胞存活率的作用(P<0.01)。空斑减少试验结果显示:2种模式下EGCG治疗指数(TI)分别为39.31及43.48,能有效抑制流感病毒,符合CPE法的结果。【结论】EGCG具有一定的体外抗甲型流感病毒FM1株的作用。Objective To investigate the inhibitory effect of EGCG, the main components of tea catechin, on influenza A virus FM1 strain infection and on viral replication. Methods The cells were cultured for testing anti viral titer. Cytopathic effect （CPE） test and the plaque reduction test were adopted to evaluate invitro anti influenza virus actions by experimental treatment and protection strategies. Results The results of CPE and methyl thiazolyl tetrazolium （MTT） assay showed that EGCG at the concentrations of 4-32 mg/mL had obvious effect on inhibiting influenza virus and increasing cell survival rate （ P 〈 0.01 ） by the treatment and protection strategies. The results of plaque reduction test showed that therapeutic index （TI） of EGCG was 39.31 and 43.48 by the treatment and protection strategies respectively, indicating that EGCG had certain antiviral effect, which was as same as the results of CPE. Conclusion EGCG exerts certain antiviral effect on influenza A virus FM1 strain in vitro.

关 键 词：茶多酚/药理学 流感病毒/中药疗法 细胞培养 体外的 

分 类 号：R285.5[医药卫生—中药学]