Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats  

作　　者：Jiaxue Zhu Jinbao Qin Zunli Shen James D. Kretlow Xiaopan Wang Zhangyin Liu Yuqing Jin 

机构地区：[1]Department of Plastic and Reconstructive Surgery, Shanghai First People＇s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China [2]Stem Cell and Tissue Engineering Research Center, Shanghai First People＇s Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200080, China [3]Department of Bioengineering, Rice University, Houston 77251-1892, TX, USA

出　　处：《Neural Regeneration Research》2012年第4期256-260,共5页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China, No. 30872630, 81000522 and 30973050;Shanghai Shenkang Laboratory Funding, No. SHDC12007706

摘　　要：In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.

关 键 词：DISPASE differential detachment PURIFICATION Schwann cell neural regeneration 

分 类 号：Q510.3[生物学—生物化学] Q421