The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells Superior effects over original formula of Buyang Huanwu decoction  

作　　者：Jinghui Zheng Yi Wan Jianhuai Chi Dekai Shen Tingting Wu Weimin Li Pengcheng Du 

机构地区：[1]Grade Three Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine of China, Ruikang Hospital, Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China [2]Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China

出　　处：《Neural Regeneration Research》2012年第4期261-267,共7页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Anhui Province (Role of substance P in electroacupuncture for focal brain ischemia in rats), No. 050431003;the National Natural Science Foundation of China (Target point and signal transduction pathway of Yangxin Tongmai active principle region for BMSCs), No. 81102595

摘　　要：The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction （active principle region of decoction for invigorating yang for recuperation）. After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula.The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction （active principle region of decoction for invigorating yang for recuperation）. After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula.

关 键 词：active principle region bone marrow-derived mesenchymal stem cells Buyang Huanwu decoction differentiation nerve cells 

分 类 号：Q813[生物学—生物工程] TQ461[化学工程—制药化工]