Increased CD133^+ cell infiltration in the rat brain following fluid percussion injury  

作　　者：Ming Wei Ziwei Zhou Shenghui Li Chengwei Jing Dashi Zhi Jianning Zhang 

机构地区：[1]Tianjin Neurological Institute, General Hospital, Tianjin Medical University, Tianjin 300052, China [2]Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin 300070, China

出　　处：《Neural Regeneration Research》2012年第4期278-282,共5页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China, No. 30772229;the National Basic Research Program of China (973 Program), No. 2005CB522600;the Science Foundation of Tianjin Bureau of Public Health, No. 2011KZ96

摘　　要：The prominin-1/CD133 epitope is expressed in craniocerebral trauma in animal models of fluid undifferentiated cells. Studies have reported that percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133＋ cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133＋ cells were mainly distributed in hippocampal CA1 3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression.The prominin-1/CD133 epitope is expressed in craniocerebral trauma in animal models of fluid undifferentiated cells. Studies have reported that percussion injury induces production of a specific stem cell subgroup. It has been hypothesized that fluid percussion injury induces CD133＋ cell infiltration in the brain tissue. The present study established a traumatic brain injury model through fluid percussion injury. Immunohistochemical staining showed significantly increased CD133 antigen expression in the rat brain following injury. CD133＋ cells were mainly distributed in hippocampal CA1 3 regions, as well as the dentate gyrus and hilus, of the lesioned hemisphere. Occasional cells were also detected in the cortex. In addition, reverse transcription-PCR revealed that no change in CD133 mRNA expression in injured brain tissue. These results suggested that fluid percussion injury induced CD133 antigen expression in the brain tissues as a result of conformational epitope changes, but not transcriptional expression.

关 键 词：prominin-1 IMMUNOHISTOCHEMISTRY reverse transcription-PCR traumatic brain injury neural regeneration 

分 类 号：Q427[生物学—神经生物学] TH137[生物学—生理学]