Electroacupuncture effects on cortical neurons, as well as Janus kinase 2-signal transducer and activator of transcription 3 signal transduction pathway, in a rat model of cerebral ischemia  

作　　者：Rong Liu Nenggui Xu Wei Yi Kangbai Huang 

机构地区：[1]Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China

出　　处：《Neural Regeneration Research》2012年第6期457-462,共6页中国神经再生研究（英文版）

基　　金：supported by the National Basic Research Program of China (973 Program), No.2010CB530500;the National Natural Science Foundation of China, No. 30973788;Guangdong Province Science and Technology Projects, No. 2010B031500016;the Natural Science Foundation of Guangdong Province, No.9351040701000001

摘　　要：The present study established a model of focal cerebral ischemia through heat-coagulation induced occlusion of the middle cerebral artery. Following electroacupuncture at Baihui （GV20） and Dazhui （GV14）, or intracerebroventricular infusion of AG490, a Janus kinase 2 phosphorylation inhibitor, the amount of necrotic or degenerated neurons in the ischemic cerebral cortex decreased, neuronal swelling was ameliorated, and expression of phosphorylated Janus kinase 2 and signal transducer and activator of transcription 3 decreased Results confirmed that electroacupuncture promoted neuronal repair in the cerebral cortex by reducing expression of phosphorylated Janus kinase 2 and signal transducer and activator of transcription 3, as well as weakening the phosphorylated activation, thereby blocking abnormal activation of the Janus kinase 2- signal transducer and activator of transcription 3 signal transduction pathway.The present study established a model of focal cerebral ischemia through heat-coagulation induced occlusion of the middle cerebral artery. Following electroacupuncture at Baihui （GV20） and Dazhui （GV14）, or intracerebroventricular infusion of AG490, a Janus kinase 2 phosphorylation inhibitor, the amount of necrotic or degenerated neurons in the ischemic cerebral cortex decreased, neuronal swelling was ameliorated, and expression of phosphorylated Janus kinase 2 and signal transducer and activator of transcription 3 decreased Results confirmed that electroacupuncture promoted neuronal repair in the cerebral cortex by reducing expression of phosphorylated Janus kinase 2 and signal transducer and activator of transcription 3, as well as weakening the phosphorylated activation, thereby blocking abnormal activation of the Janus kinase 2- signal transducer and activator of transcription 3 signal transduction pathway.

关 键 词：cerebral ischemia JAK2-STAT3 neurons ELECTROACUPUNCTURE AG490 

分 类 号：Q25[生物学—细胞生物学] Q2