甲氧滴滴涕染毒对孕鼠胎盘组织ERα表达的影响  

Effects of methoxychlor exposure on the expression of ERα mRNA in mice placenta during early pregnancy

作　　者：王晓蓉 苑洪菲 车丽萍 

机构地区：[1]黑龙江省武警总队医院妇产科,黑龙江哈尔滨150076

出　　处：《哈尔滨医科大学学报》2012年第1期40-42,共3页Journal of Harbin Medical University

摘　　要：目的探讨孕早期持久性暴露有机污染物甲氧滴滴涕(methoxychlor,MXC)对孕鼠胎盘组织雌激素受体α(estrogen receptorα,ERα)表达的影响。方法孕鼠随机分为3组,每组10只,其中孕鼠皮下注射MXC为染毒组,皮下注射芝麻油为正常对照组,皮下注射雌二醇(estradiol,E2)为阳性对照组。应用半定量逆转录-聚合酶链反应(RT-PCR)技术检测各组孕鼠胎盘组织中ERα的表达强度。结果孕鼠MXC染毒组ERαmRNA(0.68±0.11),与正常对照组(0.49±0.09)比较,有显著性差异(P<0.05);与阳性对照组(0.60±0.10)比较,差异无显著性(P>0.05)。免疫组化结果显示,MXC染毒孕鼠胎盘组织ERα阳性表达明显高于正常对照组。结论 ERα中的高表达可能是MXC影响胚胎发育的毒性作用机制之一。Objective To investigate the effects of methoxychlor （MXC） exposure on the ex- pression of estrogen receptor ot （ERa） mRNA in mouse placenta during early pregnancy. Methods Pregnant mice were randomly divided into three groups with 10 for each, in which subcutaneous injection of MXC as the exposure group, subcutaneous injection of sesame oil as the normal control group, and subcutaneous injection of estradio] （E2 ） as a positive control group. Placenta tissues were obtained from each group for analysis of ERoL mRNA expression by using semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results The expression of ERa mRNA were significantly different between the MXC exposure group （0.68 ± 0. 11 ） and the normal control group （0. 49 ± 0. 09 ）, P 〈 0. 05 ; and was not different between the MXC exposure group and the positive group （0. 60 ± 0. 10）, P 〉 0. 05. Immuno- histochemistry showed that the expression of ERa in pregnant mice placenta exposed to MXC was significantly higher than that in the normal control group. Conclusion The high expres- sion of ERa may be one of the toxic mechanisms involved in the effects of MXC on embryonic development.

关 键 词：环境污染 甲氧滴滴涕 雌激素受体Α 

分 类 号：R714.56[医药卫生—妇产科学]