氧化震颤素在精氨酸加压素引起大鼠低温中的作用与对行为性体温调节反应的影响  

Role of oxotremorine in arginine vasopressin-induced hypothermia and its effects on behavioral thermoregulatory response in rats

作　　者：沈字玲[1] 杨永录[1] 孙兵[1] 唐瑜[1] 王念[1] 

机构地区：[1]成都医学院生理教研室,体温调节实验室,四川成都610083

出　　处：《中国应用生理学杂志》2012年第2期107-112,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30870901);成都医学院资助项目(CYZ07-009)

摘　　要：目的:研究氧化震颤素在精氨酸加压素(AVP)引起低温中的作用及其对行为性体温调节反应的影响。方法:无线遥控测温技术记录成年雌性SD大鼠体核温度(Tc)、棕色脂肪组织(BAT)温度和活动的变化。用无线遥测温度梯度仪记录大鼠行为性体温调节反应。分别观察AVP(10μg/kg)和氧化震颤素(0.25 mg/kg)对大鼠Tc、活动、BAT温度(TBAT)、理毛活动和行为性体温调节反应的影响。结果:AVP和氧化震颤素均能引起Tc和TBAT降低,理毛活动增加,引起低温反应的同时动物选择较低环境温度。氧化震颤素能使AVP引起的Tc和TBAT降低,以及理毛活动的增加更明显,并持续更长时间。注射氧化震颤素后立即注射AVP动物亦选择较低环境温度,但与AVP比较无明显差异。结论:AVP引起的低温与体温调定点下移、抑制BAT产热和增加理毛活动有关。氧化震颤素可能通过影响BAT产热和行为性体温调节参与外周给AVP引起的低温过程。Objective： To investigate the role of oxotremorine in arginine vasopressin（AVP）-induced hypothermia and its effects on the behavioral thermoregulatory response.Methods： Core temperature（Tc）,brown adipose tissue（BAT） temperature and motor activities were monitored in undisturbed female SD rats using radiotelemetry.The behavioral thermoregulatory response was monitored in rats using radiotelemetric temperature gradient apparatus.Effect of AVP（10 μg/kg） and oxotremorine（0.25 mg/kg） on Tc,motor activities,BAT temperature（TBAT）,grooming activities and the behavioral thermoregulatory response were observed in rats.Results： Administration of AVP and oxotremorine caused a significant drop in Tc,TBAT,and an increases in grooming activities,respectively.The hypothermic responses were accompanied with a preference for cooler ambient temperature.Oxotremorine augmented the reduction of Tc,TBAT,and the elevation of grooming activities resulting from AVP,and lasting a longer time.Administration of oxotremorine followed immediately by AVP injection in rats was also shown to induce a preference for cooler ambient temperature,but there was no significant difference compared with AVP.Conclusion： AVP-induced hypothermia was related with the set point temperature reduction,inhibiton of BAT thermogenesis and an increases in grooming activities.Oxotremorine could participate in peripheral AVP-induced hypothermia by affecting BAT thermogenesis and behavioral thermoregulation.

关 键 词：精氨酸加压素 氧化震颤素 低温反应 棕色脂肪组织 行为性体温调节 

分 类 号：R339.6[医药卫生—人体生理学] R364.6[医药卫生—基础医学]