肠淋巴液引流对失血性休克大鼠红细胞流变性的影响  

The effects of mesenteric lymph drainage on erythrocyte rheology in rats with hemorrhagic shock

作　　者：赵自刚[1] 牛春雨[1] 李志鹏[1] 张敏[1] 许帼杰[1] 姜华[1] 张静[1] 

机构地区：[1]河北北方学院微循环研究所,张家口075029

出　　处：《中国应用生理学杂志》2012年第2期149-153,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30370561);河北省自然科学基金资助项目(C2004000649);河北省科技支撑计划(11276103D-84)

摘　　要：目的:观察肠淋巴液引流对失血性休克大鼠红细胞流变性指标以及血液黏度的作用。方法:Wistar雄性大鼠均分为假休克组、休克组(复制失血性休克模型)、引流组(复制失血性休克模型,自低血压1 h引流休克肠淋巴液)。在低血压3 h或相应时间,经腹主动脉取血,检测红细胞参数、红细胞电泳、红细胞沉降率(ESR)以及血液黏度,计算红细胞聚集指数、红细胞变形指数。结果:与假休克组比较,休克组红细胞数量、红细胞比积(HCT)、血红蛋白(Hb)、平均红细胞血红蛋白浓度(MCHC)、红细胞电泳率与迁移率、红细胞变形指数、全血黏度、全血低切与高切相对黏度和还原黏度显著降低,休克组平均红细胞体积、红细胞电泳时间、ESR、血沉方程K值与校正K值、红细胞聚集性指数、血浆黏度显著升高;引流组MCHC、红细胞电泳率与迁移率、全血黏度、全血低切与高切还原黏度均显著降低,引流组红细胞体积分布宽度(RDW-SD)显著增加。同时,引流组HCT、RDW-SD、红细胞变形指数、全血黏度、全血低切与高切相对黏度显著高于休克组;ESR、血沉方程K值与校正K值、红细胞聚集性指数、血浆黏度显著低于休克组。结论:休克肠淋巴液引流可改善失血性休克大鼠红细胞流变行为,从而改善血液流变性。Objective： To observe the effects of mesenteric lymph drainage on erythrocyte rheology and blood viscosity in hemorrhagic shock rats.Methods： Wistar rats were radomly divided into sham-shock group,shock group（establishing hemorrhagic shock model）,drainage group（establishing hemorrhagic shock model puls drainaging shock mesenteric lymph from hypotension 1 h）.At 3 h of hypotension or corresponding time,blood samples were harvested from the abdominal aorta for determining the erythrocytic parameters,erythrocyte electrophoresis,erythrocyte sedimentation rate（ESR） and blood viscosity,and the erythrocytes aggregation index and erythrocyte deformability index were calculated.Results： Compared with the sham-shock group,the red cell contents,hematocrit（HCT）,hemoglubin （Hb）,mean corpuscular hemoglubin concentration（MCHC）,erythrocyte electrophoretic rate and mobility,erythrocyte deformability index,whole blood viscosity,whole blood relative or reduced viscosity at low and high shear rates in shock group were observably lower,and mean corpuscular volume,electrophoretic time of erythrocyte,ESR,K value of equation and K value of emendation,erythrocytes aggregation index,plasma viscosity in shock group were increased markedly;the MCHC,erythrocyte electrophoretic rate and mobility,whole blood viscosity,whole blood relative viscosity at low and high shear rates in drainage group were reduced,and the red blood cell volume distribution width-SD（RDW-SD） was increased remarkably.At the same time,in drainage group,the HCT,RDW-SD,erythrocyte deformability index,whole blood viscosity and relative viscosity at low and high shear rates were higher,the ESR,K value of equation and K value of emendation, erythrocytes aggregation index,plasma viscosity were lower than that of shock group.Conclusion： The results indicate that the mesenteric lymph drainage could improve the erythrocyte rheological behavior,as a result,improve the hemorrheological properties in hemorrhagic shock rats.

关 键 词：肠淋巴液 引流术 失血性休克 红细胞流变性 血黏度 

分 类 号：R331.4[医药卫生—人体生理学] R364.1[医药卫生—基础医学]