西藏当雄牦牛皮蝇蛆病病原的分子分类鉴定  

Molecular classification of hypodermosis' pathogens from yak in Dangxiong County of Tibet Autonomous Region

作　　者：刘建枝[1] 色珠[1] 关贵全[2] 夏晨阳[1] 次仁多吉[1] 拉巴次旦[1] 罗建勋[2] 殷宏[2] 佘永新[1] 姚海潮[1] 曾江勇[1] 鲁志平[1] 杨德全[1] 吴金措姆[1] 四郎玉珍[1] 

机构地区：[1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850009 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2012年第3期238-242,共5页Chinese Veterinary Science

基　　金：西藏自治区科技厅重点科研项目

摘　　要：2011年在西藏当雄县感染皮蝇蛆牦牛的背部皮下收集到三期幼虫113个,首先进行了形态学鉴定,之后提取DNA,用特异性引物扩增COⅠ基因,并进行测序,与GenBank中的相关序列进行了同源性比较,建立了系统发育树。结果表明,西藏当雄县感染牦牛的皮蝇蛆为牛皮蝇蛆和中华皮蝇蛆。前者(85条)占样本总数(113)的75.22%,为西藏当雄牦牛皮蝇蛆病病原的优势虫种;COⅠ基因序列显示牛皮蝇和中华皮蝇的种间差异为10.8%～11.3%,同源性为88.7%～89.2%;牛皮蝇的株间差异为0.1%～0.6%,同源性为99.4%～99.9%,中华皮蝇的株间差异为0.1%～0.4%,同源性为99.6%～99.9%。说明COⅠ基因是牦牛皮蝇蛆病病原种类分子分类鉴定的可靠靶基因。A totol of 113 third stage larvae of Hypoderma spp.were collected at Dangxiong County of Tibet in 2011 and morphologically characterized.Cytochrome oxidaseⅠ（COⅠ） gene was amplified,sequenced and compared with COⅠ sequences of other species in the GenBank,with a phylogenetic tree constructed.Result indicated that the specimens from the County were Hypoderma bovis and H.sinence.The H.bovis was 75.22%（85/113） of total specimen and it was the prevalent species in Dangxiong County.The interspecific difference of COⅠ sequence between H.bovis and H.sinence was 10.8%-11.3%,and the homology was 88.7%-89.2%.The intraspecific difference and the homology of H.bovis was 0.1%-0.6% and 99.4%-99.9%,respectively,as well as 0.1%-0.4% and 99.6%-99.9% of H.sinence,respectively.The COⅠ was therefore confirmed to be reliable target gene for the identification of hypodermosis＇ pathogen.

关 键 词：西藏 牛皮蝇 中华皮蝇 COⅠ基因 分子分类 系统发育树 

分 类 号：S852.743[农业科学—基础兽医学]