超声破坏靶向VEGFR2微泡治疗皮下结肠癌移植瘤的实验研究  

Study on hypovascular tumor＇s treatment with targeted microbubbles combined with ultrasonic abrupted destroy

作　　者：黄品同[1] 叶智敏[1] 朱江[1] 张旭[1] 刘政[2] 李世岩[3] 莫国强[1] 刘学明[1] 

机构地区：[1]浙江大学医学院附属第二医院超声科,杭州310009 [2]第三军医大学附属新桥医院超声科 [3]浙江大学医学院附属邵逸夫医院超声科

出　　处：《中华超声影像学杂志》2012年第3期253-256,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：国家自然基金项目资助（81071164）

摘　　要：目的探讨靶向VEGFR2微泡结合超声辐照治疗结肠癌的效果。方法将17只VEFGR2高表达的结肠癌皮下种植瘤Balb／C裸鼠模型分为三组：A组5只，为对照组，仅接受超声造影检查和假照；B组6只，空白脂质微泡结合超声辐照；C组6只，载靶向VEGFR2单抗的脂质微泡结合超声辐照，所有模型均用红色荧光蛋白标记。空化治疗前和空化后1周分别行超声造影和荧光摄片检查，测量肿块大小、荧光面积、荧光强度及血管密度，并进行比较。结果治疗前裸鼠肿瘤长径、荧光面积、荧光强度及血管密度在各组间比较差异无统计学意义（P〉0．05）；A组假照后肿瘤长径、荧光面积、荧光强度及血管密度均比假照前增加，差异有统计学意义（P〈0．05）；B组辐照后荧光强度及血管密度均较空化前减小，差异有统计学意义（P〈O．05），而肿瘤长径和荧光面积差异不显著（P〉0．05）；C组辐照后肿瘤长径、荧光面积、荧光强度及血管密度与辐照前比较均明显减小，差异有统计学意义（P〈0．01）；治疗后A、B、C三组各参数两两比较差异有统计学意义（P〈0．05）。结论靶向VEGFR2脂质微泡能增强超声空化对结肠癌的治疗效果。Objective To assess the therapeutic efficacy of colonic tumor with targeted microbubbies encapsulated VEGFR2 monoantibody （mAb）combined with ultrasonic abrupted destroy. Methods Seventeen Balb/C nude mice with subcutaneous colonic carcinoma xenografts were divided into three groups：group A （5 mice） underwent contrast-enhanced ultrasonography （CEUS） examination and sham ultrasonic abrupted destroy;group B （6 mice） underwent lipid-microbubbles administration combined with ultrasonic irradiation;group C （6 mice） underwent VEGFR2 monoantibody-loaded microbubbles injection combined with ultrasonic irradiation. Red fluorescent protein（RFP） was labeled to all the nude mice model. Both CEUS and flurography were performed before and one week after abrupted destroy. The size, fluroscent area and fluroscent intensity（FI） and vessel density （VD） of each tumor were measured and compared. Results The parameters of length, fluroscent area, FI and VD of each tumor before abrupted destroy were no significant difference among three groups （ P 〉0.05）. Parameters of post-sham ultrasonic abrupted destroy in group A were higher than those before sham ultrasonic abrupted destroy （ P 〈0.05）. FI and VD in group B were significantly lower than those after abrupted destroy（ P 〈0.05）. There were no difference of length,fluroscent area of tumor in group B between pre- and post- ultrasonic abrupted destroy （ P 〉0.05）. Length, fluroseent area, FI and VD of each tumor in groups C were decreased significantly compared with post ultrasonic abrupted destroy （P 〈0.01）. There were significant difference of length, fluroscent area,FI and VD of each tumor among groups after ultrasonic abrupted destroy （P 〈0.05）. Conclusions VEGFR2 mAb-loaded lipid microbubble combined with ultrasonic abrupted destroy can improve the therapeutic efficacy of colonic tumor.

关 键 词：超声检查 超声疗法 微气泡 结肠肿瘤 肿瘤种植 

分 类 号：R445.1[医药卫生—影像医学与核医学]