利用甲基纤维素改良的病毒噬斑技术  

The improvements of plaque assay using methylcellulose

作　　者：盛子洋[1] 王娟[1] 吴江漫[1] 安静[1] 范东瀛[1] 

机构地区：[1]首都医科大学微生物学教研室,北京100069

出　　处：《免疫学杂志》2012年第4期363-365,368,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30872227)

摘　　要：目的探究病毒噬斑实验的方法寻找其改良之处,为实验操作提供方便。方法用甲基纤维素代替琼脂糖覆盖病毒感染后的细胞。结果改良后的新方法在进行登革病毒滴度检测时相较于传统方法,可形成均匀、清晰的噬斑,复孔重复性好,而且噬斑在实验后2年内仍保存完好,与背景形成明显对比,便于复查。结论利用甲基纤维素的病毒噬斑技术,省时省力节约成本且便于操作,是一个值得推广的有价值的实验方法。Plaque assay is widely used in virus research for its accurate measurement of viral titers since developed in 1952.However,the traditional method,in which agarose is used,demonstrated a lot of limitations during the process of experiment.In this study,agarose was replaced by methyl cellulose to improve the traditional plaque assay.After the improvement,this method became easy in application and the plaque is much clearer for observation compared with the traditional ones even two years later.Thus,plaque assay using methylcellulose is a valuable experimental method for viral research work.

关 键 词：登革病毒 噬斑实验 甲基纤维素 

分 类 号：R373[医药卫生—病原生物学]