BACE2在人体胰腺和胰岛素瘤中的表达  

Expression analysis of BACE2 in human pancreas and insulinoma

作　　者：谢建[1] 刘胜春[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《重庆医科大学学报》2012年第1期6-9,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30871295);重庆市科委自然科学基金资助项目(编号:CSTC;2009BB5065)

摘　　要：目的:研究BACE2(Beta-site APP cleaving enzyme 2,BACE2)在人体胰腺组织和胰岛素瘤中的分布和表达,探讨其在胰腺组织中的生理功能及其在2型糖尿病(Type 2 diabetes,T2DM)淀粉样蛋白沉积中的可能作用。方法:收集人体胰腺和胰岛素瘤标本,运用免疫组织化学检测BACE1(Beta-site APP cleaving enzyme 1,BACE1)、BACE2、胰岛素淀粉样多肽(Islet amyloid polypeptide,IAPP)在胰腺组织中的分布和BACE2在胰岛素瘤中的分布,运用Western blot检测BACE2在胰腺和胰岛素瘤中的蛋白表达。结果:免疫组织化学显示BACE2表达于人体胰腺中的胰岛和胰岛素瘤中,BACE1表达于胰腺胰岛中;免疫组织化学双染发现BACE2在胰岛的表达部位和IAPP在胰岛中表达部位相同,即β细胞中;Western blot检测显示BACE2在人体胰腺和胰岛素瘤中都有表达,且胰岛素瘤中表达高于对照组的胰腺组织。结论:BACE2在人体胰岛细胞和胰岛素瘤中的表达,特别是BACE2与IAPP同时表达于胰岛β细胞,可能与T2DM的淀粉样蛋白沉积相关。Objective：To determine the expression and distribution of BACE2 in human pancreas and insulinoma,and to investigate the function of BACE2 in normal pancreas as well as the possible roles of BACE2 in the amyloidogenesis of T2DM.Methods：Human pancreas and insulinoma tissues obtained from the department of general surgery,the first affiliated hospital were used in this study.We have used immunohistochemistry to detect the distribution of BACE2,BACE1 and IAPP in human pancreas and the distribution of BACE2 in human insulinoma.Western blot were applied to examine the protein levels of BACE2 in human pancreas and insulinoma.Results：Immunohistochemical staining showed that BACE2 expressed in human pancreas islets cells as well as insulinoma,BACE1 expressed in pancreas islets.Double stains indicated that BACE2 expressed mainly in islet β-cells,the same as the expression position of IAPP in pancreas.Western blot revealed that both pancreas and insulinoma expressed BACE2,and the protein level in insulinoma was higher.Conclusion：The presence of BACE2 in human pancreas and insulinoma,especially the coexpression of BACE2 and IAPP in islet β-cells suggests that BACE2 may play a potential role in the amyloidogenesis of T2DM.

关 键 词：BACE2 BACE1 胰岛素淀粉样多肽 胰腺 胰岛素瘤 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌] R587.3[医药卫生—内科学]