胎儿骨髓间充质干细胞EGF受体或IGF受体的表达与其向上皮细胞分化的关系  

The relation between the expression of EGF-R or IGF-1-R and epithelial differentiation in fetal bone marrow mesenchymal stem cells

作　　者：姜蓉[1] 段江洁[1] 田静[1] 汪维伟[1] 

机构地区：[1]重庆医科大学组织学与胚胎学教研室,干细胞与组织工程实验室,重庆400016

出　　处：《重庆医科大学学报》2012年第1期39-42,共4页Journal of Chongqing Medical University

基　　金：重庆医科大学创新基金资助项目(编号:CX200307)

摘　　要：目的:观察骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)表达表皮生长因子受体(Epidermal growth factor-receptors,EGF-R)和胰岛素样生长因子1受体(Insulin-like growth factor-1-receptors,IGF-1-R)与其向上皮细胞分化分化的关系。方法:取第3代(P3)-BMSCs分别用或不用含EGF、IGF-1的介质诱导培养;另取P5-BMSCs用EGF-R抗体或IGF-1-R抗体孵育4 h后,加入含或不含EGF、IGF-1的培养基培养。免疫组织化学SP法检测各组细胞表达EGF-R、IGF-1-R和细胞角蛋白(Cytokeratin,CK)的时间。结果:P3-BMSCs分别于无诱导培养的8～11 d、10～14 d和16～18 d首次表达EGF-R,IGF-1-R和CK;经EGF或IGF-1诱导后,EGF-R、IGF-1-R的表达较无诱导组提前2～4 d。P5-BMSCs经抗EGF-R或IGF-1-R抗体阻滞后,细胞表达CK的时间较对照晚2 d。结论:EGF-R和IGF-1-R的表达与培养的BMSCs向上皮细胞分化密切相关;体外培养的P4-BMSCs～P5-BMSCs为加入外源性EGF、IGF-1诱导其向上皮细胞分化的最佳时间点。Objective：To observe the relation between the expression of EGF-R or IGF-1-R and epithelial differentiation in bone marrow mesenchymal stem cells（BMSCs）.Methods：The cultured passage 3（P3） BMSCs were induced or not induced by EGF or IGF-1;The P5-BMSCs were cultured with antibody of EGF-R or IGF-1-R for 4 h,and then induced or not induced by EGF or IGF-1.The expression time of EGF-R,IGF-1-R and CK in these cells was detected with immunohistochemical Sp method.Results：The first expression time of EGF-R,IGF-1-R and CK in un-induced P3-BMSCs was respectively on the 8th～11th d,10th～14th d and 16th～18th d.The expression time of EGF-R or IGF-1-R,which was induced by EGF or IGF-1,was 2 d～4 d earlier than the time in un-induced cells.When P5-BMSCs were blocked by antibody of EGF-R or IGF-1-R,the expression time of CK was 2 days later than that of control.Conclusion：The expression of EGF-R and IGF-1-R in BMSCs was closely correlated with their epithelial differentiation in vitro;The passages 4～5 BMSCs were best time for the addition of exogenous EGF、IGF-1 to induce BMSCs differentiation into epithelial cell in vitro.

关 键 词：骨髓间充质干细胞 受体 分化 诱导 

分 类 号：R285.5[医药卫生—中药学]