An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents  

作　　者：Zhilan Chen Meiling Lu Dandan Zou Hailin Wang 

机构地区：[1]Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China

出　　处：《Journal of Environmental Sciences》2012年第3期541-549,共9页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 20707034, 20877091,20890112, 20921063);the National Basic Research Program (973) of China (No. 09CB421605,2010CB933500, 2011CB936001)

摘　　要：An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals. The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor, and then the reporter egfp gene under the control of recA promoter is stimulated to express as a fluorescent protein, allowing fast and sensitive fluorescence detection. Interestingly, this biosensor can be simultaneously applied for evaluation of genotoxicity and cytotoxicity. The SOS-EGFP bacterial biosensor provides a sensitive, specific and simple method for detecting known and potential DNA damaging chemicals.An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals. The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor, and then the reporter egfp gene under the control of recA promoter is stimulated to express as a fluorescent protein, allowing fast and sensitive fluorescence detection. Interestingly, this biosensor can be simultaneously applied for evaluation of genotoxicity and cytotoxicity. The SOS-EGFP bacterial biosensor provides a sensitive, specific and simple method for detecting known and potential DNA damaging chemicals.

关 键 词：bacterial biosensor detection DNA damage GENOTOXINS SOS response 

分 类 号：Q523[生物学—生物化学] TP212.3[自动化与计算机技术—检测技术与自动化装置]