西洛他唑对大鼠颈总动脉球囊损伤后内膜增生及氧化应激的影响  

Effects of Cilostazol on Oxidative Stress and Neointimal Hyperplasia after Balloon Injury in Rat Model

作　　者：苏华科[1] 宋耀明[2] 郭文昀[2] 李文军[1] 刘启亮[2] 

机构地区：[1]解放军第253医院心内科,内蒙古呼和浩特010051 [2]第三军医大学新桥医院全军心血管病研究所,重庆400037

出　　处：《现代生物医学进展》2012年第2期247-249,225,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨西洛他唑对大鼠颈总动脉球囊损伤后内膜增生的抑制作用和血管壁氧化应激的影响。方法:SD大鼠24只,随机分组:假手术组、损伤组及西洛他唑治疗组。采用球囊损伤大鼠左侧颈总动脉,于术后2周处死大鼠,取损伤血管标本,进行HE染色、免疫组化染色及原位DHE染色,检测内膜增生、平滑肌细胞增殖及血管壁局部ROS水平。结果:球囊损伤2周后,血管壁内膜显著增生,西洛他唑治疗后内膜增生显著抑制,两组相比P<0.05。PCNA免疫组化染色:假手术组未见PCNA阳性细胞,损伤组PCNA阳性细胞面积百分比明显高于西洛他唑组,主要分布于新生内膜和内弹力膜区域(P<0.05)。原位DHE染色:球囊损伤后局部ROS水平显著升高,较假手术组差异显著P<0.05,西洛他唑干预后局部ROS水平显著降低(P<0.05)。结论:新型抗血小板制剂西洛他唑可显著抑制大鼠颈总动脉球囊损伤后内膜增生及局部氧化应激,抑制局部氧化应激可能是西洛他唑抑制内膜增生的机制之一。Objective： To investigate the effect of cilostazol on oxidative stress and neointimal hyperplasia after balloon injury in rat model.Methods： Twenty-four male SPF SD rats were randomly divided into three groups： sham-operation group,injury group and cilostazol group（n=8）.Except shan-operation group,all Left common carotid artery of rats were injuried by a balloon catheter.14days later,the injured carotids were sliced,HE and immunohistochemistry stained.The level of reactive oxygen species（ROS） was examined by Dihydroethidium（DHE）.Ratios of intima/media area,PCNA positive indexes and the level of ROS were compared.Results： The ratios of intima/media area,PCNA positive indexes and the level of ROS in injury group were significantly higher than that in sham-operation group（P0.05）.Cilostazol can significantly decrease the ratios of intima/media area,PCNA positive indexes and the level of ROS（P0.05）.Conclusion： Cilostazol reduces neointimal hyperplasia by inhibition superoxode production after balloon common arterial injury in a rat model.

关 键 词：氧化应激 西洛他唑 球囊损伤 再狭窄 

分 类 号：Q953[生物学—动物学]