CaMKⅡ在大鼠异氟烷全麻机制中的作用研究  

Effect of CaMKⅡon General Anesthetics of Isoflurane in Rats

作　　者：王丛丛[1] 朱正华[1] 汤朝武[2] 石继红[2] 陈宇[1] 杨双文[1] 董海龙[1] 熊利泽[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710032 [2]第四军医大学西京医院烧伤科,陕西西安710032

出　　处：《现代生物医学进展》2012年第3期401-403,共3页Progress in Modern Biomedicine

基　　金：国家重点自然科学基金资助项目(30600582)

摘　　要：目的:探讨磷酸化钙调蛋白依赖性激酶Ⅱ(pCaMKⅡ)在异氟烷吸入全身麻醉作用中的作用及相关机制。方法:采用经侧脑室置管全脑给药,将SD大鼠随机分为溶剂对照组(control组)和CaMKⅡ抑制剂十四烷酰肉豆蔻酰-钙调蛋白自变性抑制肽(myrAIP)处理组(n=10),给药40min后行异氟烷吸入麻醉(1MAC,30min),监测动物脑电,取脑电波平稳清晰的波段,在异氟烷麻醉30min时间点进行分析,并于异氟烷麻醉30min取脑组织样本进行蛋白定量分析。结果:抑制剂组动物给药30min时脑电抑制波明显多于对照组(P<0.05);与对照组相比,抑制剂组脑组织pCaMKⅡ蛋白表达量明显降低(P<0.05)。结论:CaMKⅡ抑制剂能明显改变异氟烷全麻过程中大鼠的脑电变化及磷酸化蛋白表达变化,pCaMKⅡα参与了异氟烷全身麻醉的过程。Objective： To investigate the effectiveness and mechanism of phosphorylation of calmodulin dependent kinase Ⅱ（pCaMKⅡ）in inhalation anesthesia of isoflurane.Methods： By cathetering into the left ventricle,SD rats were randomly divided into control group and CaMKⅡ inhibitor myristoylated autocamtide-2-related inhibitory peptide（myrAIP）group（n=10）.40 minutes after of administration,rats were anesthetized with isoflurane（1MAC,30min）.The changes of EEG in 30min anesthetics were detected.And the protein levels of pCaMKⅡα were detected by Western blot in 30 min of isoflurane anesthesia.Results： Inhibitive electroencepharogram in myrAIP group was more than that in control group（P〈0.05）;Protein levels of pCaMKIIα in myrAIP was lower than that in control group（P〈0.05）.Conclusion： CaMKⅡ inhibitor can significantly change the electroencepharogram and protein levels of pCaMKⅡα;pCaMKⅡα may be involved in the process of general anesthesia of isoflurane.

关 键 词：CaMKⅡ myrAIP 异氟烷 侧脑室置管 全身麻醉 

分 类 号：Q953[生物学—动物学] R614[医药卫生—麻醉学]